FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288867
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG18000.6231241367134356Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG13380.46318894162365376Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAACCCCGATGCTGGAAGC9780.33856411428096667No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9320.3226398307871789No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9130.3160624093440926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG9100.31502386911623687No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT8500.29425306455912237No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA8280.28663710288818034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.26448157802725825No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC7630.2641353979513063No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7290.25236527536894143Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6830.23644099187515363Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC6580.2277864899763559No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6550.22674794974850018Clontech Universal Primer Mix Long (96% over 26bp)
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT6530.22605558959659638No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA6390.22120906853326963No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAA6370.22051670838136583No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6290.21774726777375056Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGG5870.2032077045837704No Hit
GCTCCAAGGACGTCGCAACCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCT5720.19801500344449174No Hit
GCCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCC5400.18693724101403067No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4990.17274385790000243No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4970.1720514977480986Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAACCCCGA4950.1713591375961948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.16858969698857953No Hit
CTCCACGGCAACGTAATTGAAAGCGGAGACGGCGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACAGCC4550.15751193455811843No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG4420.15301159357074362No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGC4400.15231923341883982No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4350.15058833303908029No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4290.14851125258336884No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCG4260.1474727123555131No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC4140.14331855144409017No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4130.14297237136813828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4060.14054911083647492No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA4040.1398567506845711No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA4040.1398567506845711No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4000.13847203038076347No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTAC3960.13708731007695585No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3930.1360487698491001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA3850.13327932924148483No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3850.13327932924148483Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3850.13327932924148483Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA3680.1273942679503024No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3540.12254774688697566No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3460.11977830627936038Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGAGCAGGTCGCCACTAGCCTTCTGGCCGGCGGCTTCCAGCATCGGGGTTCTGACGGCCTTGGCAGCTTTC3360.11631650551984132No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG3270.11320088483627412No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCTGACAA3240.11216234460841841No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG3240.11216234460841841No Hit
GAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATC3190.11043144422865886No Hit
GTCCGGAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGA3080.10662346339318787No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3050.10558492316533215Clontech Universal Primer Mix Long (96% over 25bp)
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATA3030.10489256301342834No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA2970.10281548255771687No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCCATATTTAGCTTTAGATGAAATTTACCACCCATTTTGAGCTGCATT2950.10212312240581306No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC2940.10177694232986115No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT2930.10143076225390925No Hit
GTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTC2920.10108458217795732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)