FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109541
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC5700.5203531097945062No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA3480.31768926703243533No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT2650.2419185510448143No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2450.22366054719237544Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2170.1980993417989611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGAAGCCTGCCAAGGGCGTCAGAACCCCGATGCTGGGAGGCGCCGGCCCGAAAGCTAGTGCCAACCTGCTCCA2080.18988324006536367No Hit
CCCATACTCCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGTCAGTGACGAAGCCTTGG1930.17618973717603456No Hit
GCGTTAAAGGGGGTGAAAAGCCCCCTCGCCGTAAGCGCAAGGTTTTCTACGCAACGTTCATCGGCGTAGAGTGAG1700.1551930327457299No Hit
GTCGTATATAGCTGAAGCTTAGTGGCTTTTCCTGGAACCTCGTTCAGTCACTTCACCAGCAAGCTGGCTCGATCA1700.1551930327457299No Hit
CTCTACGCCGATGAACGTTGCGTAGAAAACCTTGCGCTTACGGCGAGGGGGCTTTTCACCCCCTTTAACGCTACT1650.1506285317826202No Hit
CTCCCAAGGCTTCGTCACTGACCCCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGGCGGCGGAGTAT1510.13784792908591303No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT1430.1305447275449375No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATTTGGATTCTTCACGGCAACGTAA1420.12963182735231557No Hit
TCTCTGGAGCAGGTTGGCACTAGCTTTCGGGCCGGCGCCTCCCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTT1340.12232862581134005No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1120.10224482157365736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACTAGGGGCCGTTCTACCCGGGATCACTCCCAAGGCTTCGTCACTGACCCCCACGCCTGCCTACTCGCCAGGG1110.10133192138103542No Hit
CTCTGGAGCAGGTTGGCACTAGCTTTCGGGCCGGCGCCTCCCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTTC1110.10133192138103542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)