FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109541
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC5520.5039209063273112No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA2640.24100565085219233No Hit
GCCGAAGCCTGCCAAGGGCGTCAGAACCCCGATGCTGGGAGGCGCCGGCCCGAAAGCTAGTGCCAACCTGCTCCA2620.23917985046694848No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT2350.21453154526615606No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2050.18714453948749782Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTATATAGCTGAAGCTTAGTGGCTTTTCCTGGAACCTCGTTCAGTCACTTCACCAGCAAGCTGGCTCGATCA1920.1752768369834126No Hit
CTCCCAAGGCTTCGTCACTGACCCCCACGCCTGCCTACTCGCCAGGGCATCGTTTCTACCCTGGCGGCGGAGTAT1470.13419632831542527No Hit
CCCATACTCCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGTCAGTGACGAAGCCTTGG1450.1323705279301814No Hit
CTCTACGCCGATGAACGTTGCGTAGAAAACCTTGCGCTTACGGCGAGGGGGCTTTTCACCCCCTTTAACGCTACT1430.1305447275449375No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATTTGGATTCTTCACGGCAACGTAA1380.1259802265818278No Hit
GCACTAGGGGCCGTTCTACCCGGGATCACTCCCAAGGCTTCGTCACTGACCCCCACGCCTGCCTACTCGCCAGGG1380.1259802265818278No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT1360.12415442619658393No Hit
TCTCTGGAGCAGGTTGGCACTAGCTTTCGGGCCGGCGCCTCCCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTT1280.11685122465560839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1220.11137382349987676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTAAAGGGGGTGAAAAGCCCCCTCGCCGTAAGCGCAAGGTTTTCTACGCAACGTTCATCGGCGTAGAGTGAG1190.10863512292201094No Hit
GATCTGCACTAGGGGCCGTTCTACCCGGGATCACTCCCAAGGCTTCGTCACTGACCCCCACGCCTGCCTACTCGC1120.10224482157365736No Hit
CTCTGGAGCAGGTTGGCACTAGCTTTCGGGCCGGCGCCTCCCAGCATCGGGGTTCTGACGCCCTTGGCAGGCTTC1120.10224482157365736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)