FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382572
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCT78092.0411844045042504No Hit
CCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTG49371.2904760411112155No Hit
GTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCAC34980.9143376932969481No Hit
TAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTGGGGT27490.7185575525652688No Hit
CTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATG23380.6111267944334661No Hit
GGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTC23260.6079901299624646No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22930.5993643026672104Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTT21360.5583262758382735No Hit
GTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAAC19700.5149357506560857No Hit
ATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAACT19520.5102307539495834No Hit
CATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGT18900.49402465418274205No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17530.4582144014721412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCA17210.44984996288280377No Hit
GTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAACTAAC16300.4260635906443755No Hit
CCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCC15070.3939127798166097No Hit
GTATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCG14920.3899919492278578No Hit
GGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACC13040.34085087251550034No Hit
ACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAA12720.3324864339261629No Hit
GTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACA12550.32804282592557743No Hit
CATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCC12120.3168031115711552No Hit
TTACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTT11360.29693756992147885No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10630.27785619438955284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCA10570.27628786215405204No Hit
CCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGA10430.2726284202712169No Hit
GTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAG10170.26583231391738027No Hit
ACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACT10030.26217287203454515No Hit
GAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAG9770.25537676568070844No Hit
ACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAA9650.25224010120970697No Hit
ACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACT9440.24675093838545425No Hit
GTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATAGAGGGTT9230.24126177556120154No Hit
GCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAAC9190.24021622073753437No Hit
CTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAA8840.23106761603044657No Hit
ACGTTACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTT8820.23054483861861297No Hit
TATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGG8680.22688539673577784No Hit
CTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGA8530.22296456614702592No Hit
AGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCAT8400.21956651297010757No Hit
CCCCACAACTCCTAACTAACATAAAGTCAGCAGGATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAA8130.21250901791035412No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7750.20257624708551591Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACC7740.20231485837959914No Hit
CGGTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTC7680.20074652614409838No Hit
GTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCC7660.20022374873226478No Hit
TACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCGATTACAT7230.18898403437784259No Hit
ACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAG7090.1853245924950075No Hit
TTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTG6950.18166515061217237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.18140376190625554No Hit
CTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAA6680.1746076555524189No Hit
GGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGAGTACAGC6640.1735621007287517No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6440.1683343266104158Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6330.16545905084533108Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6150.16075405413882876Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAA5710.1492529510784898No Hit
ATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCAT5650.14768461884298903No Hit
CTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAA5590.14611628660748827No Hit
GGTATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGC5570.14559350919565467No Hit
CTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAA5330.1393201802536516No Hit
GTATCAACGCAGAGTACATGGGTAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGG5240.13696768190040043No Hit
ATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGAGT5200.13592212707673326No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5120.1338310174293989Clontech Universal Primer Mix Long (96% over 26bp)
CACGGTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTAC5080.13278546260573174No Hit
GCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCC5040.13173990778206454No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4990.13043296425248058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTATGTTAGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACG4900.12808046589922942No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4890.12781907719331265Clontech Universal Primer Mix Long (96% over 26bp)
TGTGAGTACAGCGCAAAGTGGGAGGTAAGTTACTTCTAAAGCTAAATACATCCAGAGACCGATAGCGAACAAGTA4870.12729629978147905No Hit
ATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACA4830.12625074495781186No Hit
CCCTTACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCGATT4710.12311408048681033No Hit
GAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTG4690.12259130307497675No Hit
GTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATA4610.12050019342764239No Hit
TCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAAT4610.12050019342764239No Hit
ATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCT4540.11867047248622481No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4520.11814769507439123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4510.11788630636847444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATAGAGGGTTATAGTCCC4480.11710214025072403No Hit
CCTTAGAAGTGGTGGCCATTTCTCAGGCCCCCTCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTCGAAACCA4430.11579519672114008No Hit
ATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTAT4380.11448825319155609No Hit
GTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAA4360.1139654757797225No Hit
ACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTG4350.11370408707380572No Hit
ATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACT4250.11109020001463776No Hit
GACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCC4240.11082881130872098No Hit
CTCCTAACTAACATAAAGTCAGCAGGATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCC4190.10952186777913699No Hit
ACGCAGAGTACATGGGTAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAA4130.10795353554363624No Hit
AATTCATACCCCACAACTCCTAACTAACATAAAGTCAGCAGGATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCC4110.10743075813180263No Hit
GGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAACTAA4070.10638520330813546No Hit
GGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGG4030.10533964848446829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10481687107263468No Hit
TCACGGTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTA3870.10115742918979957No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)