FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382572
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCT73021.9086603306044354No Hit
CCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTG42691.1158683855587967No Hit
GTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCAC32410.8471607958763318No Hit
TAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTGGGGT26810.7007831205629267No Hit
GGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTC21920.572964043369614No Hit
CTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATG20600.5384607341885972No Hit
ATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAACT19390.506832700772665No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19340.505525757243081Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTT19200.501866315360246No Hit
CATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGT16810.439394414646132No Hit
CTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCA16560.43285969699821214No Hit
GTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAACTAAC16440.4297230325272105No Hit
GTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAAC15700.41038026828936774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14860.388423616992357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCC14530.37979778969710276No Hit
ACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAA13540.3539203078113401No Hit
GGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACC13060.34137364992733393No Hit
GTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACA11490.3003356230983972No Hit
GTATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCG11460.2995514569806468No Hit
TTACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTT11410.2982445134510628No Hit
CCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGA11230.2935395167445605No Hit
CATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCC10930.2856978555670566No Hit
ACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACT10100.2640025929759627No Hit
GTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAG9880.2582520414457932No Hit
ACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCA9490.24805788191503822No Hit
ACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACT9430.24648954967953743No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9340.24413705132628627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATAGAGGGTT9310.24335288520853593No Hit
ACGTTACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTT9100.23786372238428322No Hit
AGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCAT9060.23681816756061605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.22714678544169462No Hit
ACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAA8510.22244178873519235No Hit
GCGCTGTACTCACAAACAACGCGACTCTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAAC8490.22191901132335873No Hit
CTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGA8370.2187823468523572No Hit
CTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACAACTCCTAA8260.21590707108727247No Hit
GAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAG8170.21355457273402131No Hit
CCCCACAACTCCTAACTAACATAAAGTCAGCAGGATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAA7860.2054515228506007No Hit
GTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCC7620.1991781939085976No Hit
CGGTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTC7530.19682569555534643No Hit
TATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGG7510.19630291814351283No Hit
TACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCGATTACAT7380.19290486496659454No Hit
GTACATGGGTAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACC6810.17800570872933721No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6690.1748690442583357Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAAAATCAACATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAA6580.17199376849325096No Hit
TGTGAGTACAGCGCAAAGTGGGAGGTAAGTTACTTCTAAAGCTAAATACATCCAGAGACCGATAGCGAACAAGTA6540.17094821366958377No Hit
TTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTG6500.1699026588459166No Hit
GGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGAGTACAGC6450.1685957153163326No Hit
ACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAG6310.16493627343349748No Hit
CTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAA6010.15709461225599367No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5950.15552628002049287Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.1534351703731585No Hit
CTTTATGTTAGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACG5750.150298505902157No Hit
ATTCAAAAGTGTATGAGTTTCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCAT5730.1497757284903234No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5690.1487301736666562Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAA5320.1390587915477348No Hit
ATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTGTGAGT5250.13722907060631725No Hit
CACGGTACTTGTTCGCTATCGGTCTCTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTAC5130.1340924061353157No Hit
ATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCCCACA4990.13043296425248058No Hit
GGTATCAACGCAGAGTACATGGGATAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGC4970.129910186840647No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4930.1288646320169798Clontech Universal Primer Mix Long (96% over 26bp)
ACACCATAGAGGGTTATAGTCCCGTGAGAAACTCATACACTTTTGAATGTTGATTTTAAAGAGTCGCGTTGTTTG4820.12598935625189506No Hit
GTATCAACGCAGAGTACATGGGTAGGCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGG4740.12389824660456071No Hit
GAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCTGAATCCTGCTG4730.12363685789864391No Hit
CCCTTACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTAATATGCTTAAGTTCAGCGGGTAGTCGATT4680.12232991436905995No Hit
ATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCCCTGTTGGTTTCTTTTCCTCCGCTTAT4670.12206852566314313No Hit
CTGGATGTATTTAGCTTTAGAAGTAACTTACCTCCCACTTTGCGCTGTACTCACAAACAACGCGACTCTTTAAAA4660.12180713695722635No Hit
TCTCACGGGACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAAT4610.12050019342764239No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4530.11840908378030802Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4520.11814769507439123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCACATGTAATCGACTACCCGCTGAACTTAAGCATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCC4520.11814769507439123No Hit
GTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATA4470.11684075154480725No Hit
CTCCTAACTAACATAAAGTCAGCAGGATTCAGAGTTGAGCTTCTCCCGTTTCAGTCGCCCTTACTAAGGGAATCC4460.11657936283889045No Hit
CCTTAGAAGTGGTGGCCATTTCTCAGGCCCCCTCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTCGAAACCA4450.11631797413297366No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4370.1142268644856393Clontech Universal Primer Mix Long (96% over 26bp)
GACTATAACCCTCTATGGTGTAACGTTCCAGAAAACTTAGTTACTCATACAAATCCTTAAGTCAAATTCATACCC4250.11109020001463776No Hit
GTATGAATTTGACTTAAGGATTTGTATGAGTAACTAAGTTTTCTGGAACGTTACACCATAGAGGGTTATAGTCCC4200.10978325648505378No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4140.10821492424955304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACTCT4110.10743075813180263No Hit
ATATTAATAAGCGGAGGAAAAGAAACCAACAGGGATTCCCTTAGTAAGGGCGACTGAAACGGGAGAAGCTCAACT4030.10533964848446829No Hit
GTCTAAACCCAGCTCACGTTCCCTTTTAACGGGTGAACAATCCAACACTTGGCGAATGCTGCTTCGCAATGATAG3960.10350992754305072No Hit
GCTCAACTCTGAATCCTGCTGACTTTATGTTAGTTAGGAGTTGTGGGGTATGAATTTGACTTAAGGATTTGTATG3910.10220298401346674No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)