FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203175
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55192.7163775070751814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32151.5823797219146056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21921.0788728928263813No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18930.9317091177556294TruSeq Adapter, Index 27 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.7190845330380214No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.3883351790328535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.28842131167712565No Hit
ATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5750.2830072597514458TruSeq Adapter, Index 27 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.2682416635905008No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.2475698289651778No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4730.2328042328042328RNA PCR Primer, Index 27 (96% over 31bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.11910914236495632No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.11271071736188014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2250.11074197120708748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10188261351052048No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)