FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279279
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58982.1118666279956604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34991.2528689948044787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.9413525542557801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.5854360693070371No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA11650.417145578435901TruSeq Adapter, Index 27 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.2696228502680116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.21949376788086467No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.20910988652924137No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.18511953995824965No Hit
ATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3310.11851947335818304TruSeq Adapter, Index 27 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3160.11314850024527444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2830.10133235939687553No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2820.10097429452268161RNA PCR Primer, Index 27 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)