FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156493
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40272.5732780379953097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26841.7150926878518529No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA19621.2537301987948342TruSeq Adapter, Index 27 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.7834216226923887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.7495542931632725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.5476283284236356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.38787677404101145No Hit
ATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5860.37445764347287097TruSeq Adapter, Index 27 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.34634136990152914No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.2952208724990894No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2530.16166857303521562RNA PCR Primer, Index 27 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1950.12460621241844683No Hit
ACACATCTCCGAGCCCACGAGACGCCTTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1850.11821615024314186RNA PCR Primer, Index 27 (100% over 29bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1156601253730199No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1760.11246509428536738No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.10415801345747096No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)