FastQCFastQC Report
Wed 8 May 2024
EGAF00002152461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98728
Total Bases7.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA39113.961388866380358No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24742.505874726521352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17451.7674823758204359No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA13471.3643545903897576No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12281.2438214083137509No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10461.05947654160927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.9429948950652297No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8760.8872862815006887No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6250.6330524268697836No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5260.53277692245361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.5196094319747184No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4910.49732598654890203No Hit
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.38996029495178675No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3400.3443805202171623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.32007130702536263No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA2990.3028522810145045No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.28360748723766305No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2720.27550441617372984No Hit
CAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.27347864840774655No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.2694271128757799No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.2420792480350053No Hit
AACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.23802771250303864No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2340.237014828620047No Hit
TATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.22891175755611376No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAG2210.2238473381411555No Hit
GGAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.21574426707722227No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1990.20156389271533912No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1930.19548658941738917No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.19042217000243092No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAA1820.184344866704481No Hit
GAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.17725467952353943No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1660.16813872457661455No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1580.16003565351268131No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.15699700186370635No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1530.15497123409772304No Hit
GTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1440.14585527915079816No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAA1390.14079085973583988No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.14079085973583988No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1370.1387650919698566No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.131674904788915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.13066202090592335No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.1276233692569484No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA1220.12357183372498177No Hit
GTATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.1185074143100235No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1150.11648164654404018No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1150.11648164654404018No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.11445587877805688No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA1120.11344299489506524No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA1120.11344299489506524No Hit
ATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.10837857548010696No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.1073656915971153No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC1040.10533992383113198No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAC1030.10432703994814035No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.10331415606514868No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAA1020.10331415606514868No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1010.10230127218215704No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1000.10128838829916538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)