FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198263
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54612.7544221564285825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34771.7537311550818862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24041.212530830260815No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22431.1313255625104028RNA PCR Primer, Index 22 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.7903643140676777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.40905262202226333No Hit
ATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7160.36113647024406975RNA PCR Primer, Index 22 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6700.33793496517252336Illumina PCR Primer Index 1 (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.3323867791771536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.28648814957909446No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.243615803251237No Hit
TATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2490.12559075571337064RNA PCR Primer, Index 22 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2220.11197248099746296No Hit
ACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2220.11197248099746296RNA PCR Primer, Index 22 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.10541553391202595No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.10238925064182423No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.10138048955175702No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)