FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163046
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA37932.3263373526489457RNA PCR Primer, Index 22 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32551.9963691228242337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20411.2517939722532292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.9359321909154472No Hit
ATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA13140.8059075352967874RNA PCR Primer, Index 22 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10980.6734295842890963Illumina PCR Primer Index 1 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.552604786379304No Hit
ACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA4410.27047581664070264RNA PCR Primer, Index 22 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.24778283429216294No Hit
TATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA4010.24594286275038948RNA PCR Primer, Index 22 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.21711664192927152No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.20669013652588838No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2920.1790905633992861TruSeq Adapter, Index 5 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.1373845417857537No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2060.12634471253511279RNA PCR Primer, Index 22 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG1790.1097849686591514No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1640.10058511095028397RNA PCR Primer, Index 22 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)