FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189764
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53342.8108598048101854No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA33161.7474336544339286RNA PCR Primer, Index 22 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32521.7137075525389431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23321.2288948377985287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.8420986066904155No Hit
TTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10470.5517379481882759Illumina PCR Primer Index 1 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA10470.5517379481882759RNA PCR Primer, Index 22 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.3730950022132754No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.33620707826563523No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.3156552349233785No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.2392445353175523No Hit
TATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3940.20762631479100355RNA PCR Primer, Index 22 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3830.20182964102780296RNA PCR Primer, Index 22 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGATATATGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2840.1496595771589975TruSeq Adapter, Index 5 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2470.13016167450095908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2340.1233110600535402No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.11698741594823044No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.10539406842182922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT1980.10434012773761092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)