FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164655
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38162.317573107406395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22681.3774255261000272No Hit
CTTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA20861.2668913789438523RNA PCR Primer, Index 22 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17671.073152956181106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.6121891227111232No Hit
ATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7540.4579271810755823RNA PCR Primer, Index 22 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.2745133764537973No Hit
TTATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4490.27269138501715706Illumina PCR Primer Index 1 (96% over 27bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.24171753059427287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.23017825149555132No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.1821991436640248No Hit
ACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2540.154261941635541RNA PCR Primer, Index 22 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACGATATATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2100.12753940056481736RNA PCR Primer, Index 22 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1760.10689016428289454No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)