FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440666
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGT187344.251292362015676No Hit
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTT124222.818915005922853No Hit
ACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCT83261.8894128432872062TruSeq Adapter, Index 18 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTC47321.0738291585917679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.973072576509193No Hit
TCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTG39520.8968243522304875Illumina PCR Primer Index 7 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCT25360.5754925499130861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.5158101600758852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.4903940853163167No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA21440.4865362882545965No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA20660.4688358076184684No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19320.43842728960255617Clontech Universal Primer Mix Long (96% over 26bp)
CATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCT19160.43479642177976063Illumina PCR Primer Index 7 (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC18550.4209537382053528No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC18300.41528050723223486No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17380.3944030172511608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16320.37034851792514056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA15810.35877512673997997No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC15020.34084771686492715No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT14810.3360822028475081No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTAT14790.33562834436965866No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA14600.331316688830089Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA13880.3149777836275093No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA13530.3070352602651441No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACA13290.30158895853095086No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA13040.2959157275578329No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT12870.2920579304961127No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC12650.2870654872397689No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATAC12440.2822999732223498No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT12290.278896034638479No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.2750382375767588No Hit
CACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTG11950.2711804405150386RNA PCR Primer, Index 18 (95% over 24bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC11870.2693650066036409No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACAC11390.2584724031352543No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTAT11260.255522323029233No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA11220.25461460607353414No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA11140.25279917216213643No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10600.2405449932602016Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10510.23850263010987915Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC10170.2307870359864387No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT9430.21399427230600954Clontech Universal Primer Mix Long (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCT9180.20832104133289156No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCT8910.20219395188192418No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT8270.1876704805907422Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7700.17473551397203324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7620.17292008006063547Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7570.17178543386601192Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTT7420.16838149528214114No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT7190.16316212278687262Clontech Universal Primer Mix Long (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7110.16134668887547485Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTA6920.1570350333359052No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCAGCTCGTATGCCGT6900.15658117485805575No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATA6690.15181566084063666No Hit
CTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGA6660.15113487312386253TruSeq Adapter, Index 18 (96% over 28bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACAT6440.14614242986751871No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTATA6320.1434192790004221Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6140.13933455269977715Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6080.13797297726622884Clontech Universal Primer Mix Long (96% over 26bp)
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5910.13411518020450863No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCACCTCGTATGCCGT5840.1325266755320356No Hit
GAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5810.13184588781526144No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTA5680.1288958077092401No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT5580.12662651531999292No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC5510.12503801064751988Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCAGCTCGTATGCCGTCTT5340.12118021358579968No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT5130.11641469956838058No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5080.115280053373757No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5020.11391847794020868No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4960.11255690250666038No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACAC4950.11232997326773567No Hit
ACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGC4740.10756445925031656TruSeq Adapter, Index 18 (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACAC4730.10733753001139185No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.10484130838321994No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCAACTCGTATGCCGT4560.10347973294967164No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACA4460.10121044056042444No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC4450.10098351132149973Clontech Universal Primer Mix Long (96% over 25bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC4450.10098351132149973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)