FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274738
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT18550.675188725258246No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG18210.6628132984880141No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14520.5285035197169667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCCGCACCCCAGCGCTGGAGGG13950.507756480719813No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC13950.507756480719813No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11880.43241197067751824Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT11560.4207645101878881No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT10030.3650750897218441No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC9510.34614796642619516No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA9340.33996025304107913No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG9000.3275848262708471No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA8940.3254009274290415No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8500.3093856692558001No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG8130.29591829306466527No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG7660.278811085470521No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA7640.2780831191899191No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCCGCACCCCAG7410.2697115069629975No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCCGCA7230.2631598104375805No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGG7180.26133989473607583No Hit
TGCCAAGGGTGTCCGCACCCCAGCGCTGGAGGGCGCCCACCTTGCGGTTAGTGCTCTCCTGCGCCAGAAGCTTCT6890.2507843836673485No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT6810.247872518544941No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG6790.24714455226433912No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCC6760.24605260284343627No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA6640.24168480515982502No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA6620.2409568388792231No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6480.23586107491500993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA6440.23440514235380616No Hit
GCGCTGGAGGGCGCCCACCTTGCGGTTAGTGCTCTCCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGCACCCAA6400.2329492097926024No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT5840.2125661539357497No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG5730.20856233939243934No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG5700.20747038997153655No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC5690.20710640683123557No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCC5500.2001907271655177No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5300.19291106435949887Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA5230.19036318237739228No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT5190.1889072498161885No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG5190.1889072498161885No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG5140.1870873341146838No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAGCCGGTTAAAAT5090.18526741841317912No Hit
CCCTAGGGGTGACCCTGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACT5000.18199157015047063No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA4950.1801716544489659No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGG4860.17689580618625744No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT4840.17616783990565557No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4820.17543987362505367Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA4810.17507589048475275No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTC4780.17398394106384993No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4710.17143605908174334Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCG4650.1692521602399377No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA4630.1685241939593358No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT4620.16816021081903484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.16706826139813202No Hit
GGAGAGCACTAACCGCAAGGTGGGCGCCCTCCAGCGCTGGGGTGCGGACACCCTTGGCAGGCTTCGGCCGTCCGG4580.1667042782578311No Hit
GTGCTCTCCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT4560.16597631197722923No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4490.16342842999512264Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA4480.1630644468548217No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC4480.1630644468548217No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT4430.161244531153317No Hit
GCCGAAGCCTGCCAAGGGTGTCCGCACCCCAGCGCTGGAGGGCGCCCACCTTGCGGTTAGTGCTCTCCTGCGCCA4350.15833266603090945No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA4350.15833266603090945No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC4320.15724071661000663No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4240.1543288514875991Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTGGCAGGCTTCGGCCGTCCGGCGCACGTTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC4230.15396486834729817No Hit
TGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT4220.15360088520699722No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCT4200.15287291892639532No Hit
CCCCAGCGCTGGAGGGCGCCCACCTTGCGGTTAGTGCTCTCCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGCA4160.15141698636519157No Hit
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG4150.15105300322489063No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4140.15068902008458968No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT4000.1455932561203765No Hit
ACTTATACCCCTCCGCCGGGGCAAAATTTACGCCCCGGCGAGTAGGCAGGCGTGGAGGCTCGTGACGAAGCCCTA3980.14486528983977462No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG3980.14486528983977462No Hit
CACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGT3970.14450130669947367No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3940.14340935727857085No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGC3890.14158944157706613No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA3740.13612969447255205No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG3720.13540172819195015No Hit
CACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTC3680.13394579563074638No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTA3650.13285384620984356No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG3630.13212587992924169No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC3620.13176189678894074No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTG3600.13103393050833884No Hit
CCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACT3600.13103393050833884No Hit
GTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGC3590.13066994736803791No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA3530.12848604852623227No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGGAGAGCACTAACCGCAAGGTGGGCGCC3530.12848604852623227No Hit
CTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG3530.12848604852623227No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGC3480.12666613282472755No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG3440.12521020026352378No Hit
GAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA3440.12521020026352378No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGAC3400.12375426770232002No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG3380.12302630142171814No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC3290.11975045315900966No Hit
CTCCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGG3260.11865850373810685No Hit
CATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAAT3230.11756655431720403No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGGAGAGCACTAACCGCAAGGTGGGCGC3200.1164746048963012No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3200.1164746048963012Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3170.11538265547539837No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3160.11501867233509745Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAGGGTCACCCCTAGGGCTTCGTCACGAGCCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGG3130.11392672291419462No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT3130.11392672291419462No Hit
CTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCT3130.11392672291419462No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC3080.11210680721268991No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC3060.11137884093208802No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTT3050.11101485779178709No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACT3020.10992290837088425No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGT3000.10919494209028237No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA2990.10883095894998143No Hit
CTCCAGCGCTGGGGTGCGGACACCCTTGGCAGGCTTCGGCCGTCCGGCGCACGTTTAACAACCAACTTAGAACTG2990.10883095894998143No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT2900.10555511068727297No Hit
CCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC2890.10519112754697202No Hit
CGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGG2880.10482714440667108No Hit
AGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGG2860.1040991781260692No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA2840.10337121184546731No Hit
GGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAGCCGGTTAAAATTCCGGCATCTGGA2830.10300722870516639No Hit
GTGACAGACCGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCC2820.10264324556486543No Hit
GTCCGCACCCCAGCGCTGGAGGGCGCCCACCTTGCGGTTAGTGCTCTCCTGCGCCAGAAGCTTCTCCCCAAGGCC2800.10191527928426354No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAA2780.10118731300366166No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT2780.10118731300366166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)