FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538975
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.5762790481933299No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG23680.4393524746045735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19660.3647664548448444Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.2916647339858064No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14080.2612366065216383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.25771139663249687No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9470.17570388236931211Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGCCCAGGCGACTGTTTAGCAAAAACACAGCTCTATGCTAAATCGAAAGATGACGTATATGGGGTGACAC8510.1578922955610186No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8510.1578922955610186Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7770.14416253072962568Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7050.13080384062340553Clontech Universal Primer Mix Long (96% over 26bp)
TCACAGTACTATGCGCTATCGGTCACTAAGGAGTATTTAGCCTTGGGAGGTGGTCCTCCCGACTTCCCACAAGGT6670.1237534208451227No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6570.12189804721925877Clontech Universal Primer Mix Long (96% over 26bp)
TCGTTACACCATTCGTGCGGGTCAGAACTTACCTGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC6490.12041374831856765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.11187902963959367No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5820.10798274502527946Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5690.10557075931165638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCTCTATCGGTCACTAAGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCCCACAGGGTTTCTCGTGTC5540.10278769887286052No Hit
TTCCAGCACCGGGCAGGTGTCACCCCATATACGTCATCTTTCGATTTAGCATAGAGCTGTGTTTTTGCTAAACAG5470.1014889373347558No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5440.10093232524699661Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)