FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541646
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG105481.9473973776230231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG84411.5583979204129634Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT68251.260048075680426Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA41880.7731987312746702Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40190.7419975408292501Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG37400.6904878832300063Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC33270.6142388201888318Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG31930.589499414746901Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30280.5590367140161655Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT24330.4491863689568464No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT23790.43921675780860564Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT22160.4091233019352123No Hit
GTACATGGGTAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCGATTTCTCTACGG21140.3902918142107576No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21130.39010719178208647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG20320.37515277505972533Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC19810.36573703119749795Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTATACGGTTAACCTTGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACGGAACAA19140.3533673284765326No Hit
GTGGTACTAAGCATACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG19030.35133648176115023No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT18300.3378590444681582Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16700.30831945588077825Clontech Universal Primer Mix Long (96% over 26bp)
TTATAGGTCATTGCATACTGAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAG16530.3051808745933691No Hit
GGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATTCCCAAAG16500.30462700730735576No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC16450.3037038951640001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGTAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGTCGCCCTATTCGGACTC16440.30351927273532897No Hit
GGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCGATTTCTCTACGGCTCCCCTATACGGTTAACCTTGCCAC14110.26050224685495693No Hit
GTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCG13790.2545943291374809No Hit
CCCCTATACGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACGGAACAA13170.24314773855987123No Hit
GAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGA13110.24204000398784448No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC12800.2363167086990396No Hit
ATTACATACAGGCTCATCCCATTTCGCTCGCCACTACTTTGGGAATCTCGGTTGATTTCTTTTCCTGCAGCTACT12190.225054740550101No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12160.2245008732640876Clontech Universal Primer Mix Long (96% over 26bp)
ATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTTCAC11790.21766984340325599No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11600.21416201725850464Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCGATTTCTCTACGGCTCCCCTATACGGTTAACCTCGCCAC11250.20770023225501524No Hit
ATTGCATACTGAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAA11130.20548476311096175No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10960.20234618182355266Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10770.19883835567880126Clontech Universal Primer Mix Long (96% over 26bp)
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCC10330.1907149688172718No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG9720.1794530006683332Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG9710.17926837823966205No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT9580.17686828666693744Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG9410.1737297053795283No Hit
GAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCAC9280.1713296138068037No Hit
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCTCGTGTC9190.16966801194876358No Hit
GCGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG9110.16819103251939457No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG9100.16800641009072345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAAGCATACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGGCTA8910.1644985839459721No Hit
TATCAACGCAGAGTACATGGGTAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCG8780.16209849237324747No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT8690.16043689051520735Clontech Universal Primer Mix Long (96% over 26bp)
GTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTG8590.1585906662284961No Hit
ACGCAGAGTACATGGGTAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGTCGCCCTATTCGGACTCGATTTC8500.15692906437045598No Hit
GTATCAACGCAGAGTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGA8450.15600595222710034No Hit
CCTATGTATTCAGTATGCAATGACCTATAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTT8350.15415972794038912No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8180.15102114665297997Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTAATAGTCGATCGGATAGTGGAAGCCTCTGGAAATAGGCGCCATAGAGGGTGATAGCCCCTTACACGAAA8070.14899029993759763No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA7820.1443747392208195Clontech SMART CDS Primer II A (96% over 26bp)
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTGCATACA7700.142159270076766No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC7700.142159270076766No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA7580.1399438009327125Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTAATAGTCGATCGGATAGTGGAAGCTTCTGGAAATAAGCGCCATAGAGGGTGATAGCCCCTTACACGAAA7500.13846682150334352No Hit
TAGTACCACCGTTCAGATTTCGTGTAAGGGGCTATCACCCTCTATGGCGTCTATTTCCAGAGACTTCCACTATCC7420.13698984207397452No Hit
GAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCG7420.13698984207397452No Hit
GTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGC7390.13643597478796116No Hit
ATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTG7210.1331127710718809No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7200.1329281486432098Clontech Universal Primer Mix Long (96% over 26bp)
CCCATTATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTA7170.1323742813571964No Hit
GTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTC6940.12812796549776054No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC6910.12757409821174714Clontech SMART CDS Primer II A (96% over 26bp)
CTGAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGA6900.12738947578307605No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG6900.12738947578307605Clontech Universal Primer Mix Long (96% over 26bp)
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC6900.12738947578307605No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC6810.12572787392503593Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGTAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGTCGCCCTATTCGGACT6790.12535862906769366No Hit
CTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCA6750.12462013935300917No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT6740.12443551692433803Clontech Universal Primer Mix Long (96% over 25bp)
ATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATTACGAGTATT6670.12314315992364017No Hit
TTATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTTC6570.12129693563692892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6530.12055844592224442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATGTAATAGTCGATCGGATAGTGGAAGTCTCTGGAAATAGACGCCATAGAGGGTGATAGCCCCTTACACGAAA6350.11723524220616417No Hit
ATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGG6320.1166813749201508No Hit
GTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAG6310.11649675249147967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.11317354877539944No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT6120.1129889263467283No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6070.1120658142033727Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCGGGA6020.11114270206001706No Hit
GTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCCGG6000.1107734572026748No Hit
TAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATTCCCAA5970.11021958991666142No Hit
TAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGC5950.10985034505931919No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA5900.10892723291596355No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTT5890.10874261048729245No Hit
ATTATACAAAAGGTACGCAGTCACGGAACAAGTCCGCTCCTACTGTTTGTATGCACACGGTTTCAGGATCTATTT5780.10671176377191006No Hit
GAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCCGGCCTTTATAGGTCATTGCATACTGAA5750.10615789648589669No Hit
GTATGTGATAGTCGATCGGATAGTGGAAGTCTCTGGAAATAGACGCCATAGAGGGTGATAGCCCCTTACACGAAA5720.10560402919988332No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT5700.10523478434254106Clontech Universal Primer Mix Long (96% over 26bp)
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTG5620.10375780491317207No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG5530.10209620305513195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5500.10154233576911859Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5490.10135771334044744Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)