FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002152900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953098
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGGAAGCAGCTCGAGCAGTCCGCCGACAG57770.6061286457426204No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG40980.4299662783890009No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTG33640.3529542607370911No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG32690.3429867652644324No Hit
GGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT30850.3236813003489673No Hit
AGGTAGGACGGCGCGGCTGCTTTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC28120.2950378659906956No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT26140.2742635070055755No Hit
GTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACG24240.2543285160602582No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGAGGCCTCTAATCATTGGCTTTACCTG24130.25317438500552936No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCATTCGCGAGTTCTATCAGGTAAAGCC23720.24887262380153982No Hit
ATCCTAAGAGACGGGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCC22000.23082621094577893No Hit
GCGCTGAAGCGCGTGACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTC21900.22977700089602537No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGG21240.222852214567652No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCG20380.21382900813977154No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTA20230.21225519306514126No Hit
GAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACAA19800.20774358985120103No Hit
TTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGA19010.19945483045814807No Hit
CCCTGGAATCGACTCAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGC18720.1964121213138628No Hit
ATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTTCCT18690.19609735829893674No Hit
TCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGAAA18630.1954678322690846No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGAC18520.1943137012143557No Hit
GAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTGGCGTGAGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTG18470.19378909618947893No Hit
GCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT18080.18969717699544014No Hit
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCG17990.18875288795066195No Hit
GCCCTGGCTCACGCCACAGGTTTTGCAGCGACCGCTGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGAC17620.18487081076657386No Hit
GTGGTAGGCAGCGCGCGCCGTCACGGCGTGCTTCGGCACCTGCGCGCTCCAGGCGTCGGCCTGCGGGCCCCCCAT17220.18067397056755968No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC17020.1785755504680526No Hit
AGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA16900.17731649840834834No Hit
GTGCCCAACTACGCGCTAACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA16730.1755328413237673No Hit
GTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGAT16650.1746934732839645No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCG16640.17458855227898915No Hit
AACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG16640.17458855227898915No Hit
GTCCGCCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTT16370.17175568514465459No Hit
CCACAAAACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGATTCCAGGGTGGGCAGGCTGT15660.16430629379140443No Hit
ACCTCCGACTGAGTCGATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGA15550.16315216273667554No Hit
CCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCC15270.16021437459736565No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTATACCCGGCCG15230.1597946905774642No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC15030.15769627047795715No Hit
GTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCC14990.15727658645805573No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGATCTAGG14630.15349943027894297No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA14490.15203053620928803No Hit
GTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACAC14460.15171577319436197No Hit
ATCTAGGTTAGCGCGTAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA14310.15014195811973166No Hit
GGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGT13550.14216796174160473No Hit
TCTCCGGACTTCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGC13130.1377612795326399No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT12880.13513825440825603No Hit
GATGTGGAACGGCCAAAAGCCGGTCCGCCGATCGGCTCGGGGTGCGGACCGACGCGGATTGGGAGGGCGGCTAAA12740.13366936033860108No Hit
CCCCTAGCGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCC12700.13324967631869966No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTATACCCGGC12660.13282999229879822No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTA12660.13282999229879822No Hit
GTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG12490.1310463352142172No Hit
TCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCA12420.13031188817938974No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAA12350.12957744114456227No Hit
GTCTTAATCGACCAACACCCTTTGTGGGATCTAGGTTAGCGCGTAGTTGGGCACCGTAACCCGGCTTCCGGTTCA12140.12737410004007982No Hit
ATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGAAGCGATACTGACGTGCAAATCGTTCGTCT12050.12642981099530165No Hit
GAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGAC11940.12527567994057273No Hit
GTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTGGCGTGAGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTGGT11860.12443631190076991No Hit
GGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCC11710.1228624968261396No Hit
GCTCAACAAAGCAGCCGCGCCGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGAGGCC11680.12254773381121353No Hit
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGTCGCCGCGTGCCGGCCG11660.12233789180126284No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGG11610.12181328677638606No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGAC11590.12160344476643535No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACA11590.12160344476643535No Hit
GTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGA11470.1203443927067311No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCC11450.1201345506967804No Hit
AACCCGTACGGCGCAAGGAAGCTAATTGGCGGGATCCCCTAGCGGGTGCACCGCCGACCGACCTTGATCTTCTGA11340.11898041964205151No Hit
ACGTTAGGAAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT11280.11835089361219937No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTC11080.1162524735126923No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAA11070.11614755250771694No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAATCGACT10960.11499342145298805No Hit
GGTTAGTCGATCCTAAGAGACGGGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGT10840.1137343693932838No Hit
TCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGC10790.11320976436840703No Hit
GCCTACCACGATCGAGGGGACGACGCTCCCGGAAAACCGGGGTTTAGCCGCCCTCCCAATCCGCGTCGGTCCGCA10710.11237039632860418No Hit
ACCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTC10660.11184579130372743No Hit
CCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGG10620.111426107283826No Hit
CTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTC10530.11048181823904782No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTC10370.10880308215944216No Hit
CCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGGAAGCAGCTCGA10350.10859324014949145No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGA10290.10796371411963931No Hit
CCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTCAGCGCCATCCATTTT10240.10743910909476256No Hit
GCACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGA10060.10555053100520617No Hit
CTCCAGGCGTCGGCCTGCGGGCCCCCCATTCGGCCCGTCTTGTAACACGGACCAAGGAGTCTGACATGTGTGCGA9940.10429147894550195No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAGTATTTAGCCTTGGACGGAATTTACCGCCCACTTGGGGCTGCATTCC9870.10355703191067445No Hit
TACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCG9640.1011438487962413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)