FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002152901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953098
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGGAAGCAGCTCGAGCAGTCCGCCGACAG34660.36365620324457715No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG29010.3043758354335021No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTG28640.30049375824941404No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG23200.2434167315428214No Hit
AGGTAGGACGGCGCGGCTGCTTTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC20550.21561266522435257No Hit
GGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT20390.2139339291447469No Hit
GCGCTGAAGCGCGTGACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTC18060.18948733498548942No Hit
GTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACG16970.17805094544317582No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT16900.17731649840834834No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGAGGCCTCTAATCATTGGCTTTACCTG16440.17249013217948206No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC16040.1682932919804679No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCATTCGCGAGTTCTATCAGGTAAAGCC15320.16073897962224243No Hit
ATCCTAAGAGACGGGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCC14680.15402403530381975No Hit
CCCTGGAATCGACTCAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGC14670.15391911429884442No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTA14530.15245022022918944No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT14090.14783369601027385No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCG14020.1470992489754464No Hit
GAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTGGCGTGAGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTG13320.1397547786271716No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCG13040.1368169904878617No Hit
AACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG12740.13366936033860108No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTATACCCGGCCG12130.12726917903510446No Hit
TTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGA12110.12705933702515376No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGG11760.12338710185101638No Hit
GCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT11650.12223297079628748No Hit
GTGCCCAACTACGCGCTAACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA11490.12055423471668181No Hit
GCCCTGGCTCACGCCACAGGTTTTGCAGCGACCGCTGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGAC11290.11845581461717473No Hit
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCG11100.11646231552264301No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTA11010.11551802647786483No Hit
GTGGTAGGCAGCGCGCGCCGTCACGGCGTGCTTCGGCACCTGCGCGCTCCAGGCGTCGGCCTGCGGGCCCCCCAT10920.11457373743308662No Hit
GTCCGCCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTT10910.11446881642811128No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA10880.11415405341318521No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGAC10870.11404913240820987No Hit
ATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTTCCT10860.1139442114032345No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC10590.11111134426889996No Hit
GTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCC10360.10869816115446682No Hit
CCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCC10280.10785879311466395No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAA10230.1073341880897872No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGATCTAGG9970.10460624196042799No Hit
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGTCGCCGCGTGCCGGCCG9870.10355703191067445No Hit
TCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGAAA9590.10061924377136454No Hit
GAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACAA9570.10040940176141383No Hit
GAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGAC9570.10040940176141383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)