FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222760
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52062.3370443526665468No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA37081.6645717363979171TruSeq Adapter, Index 2 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32681.4670497396300952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.9683066977913448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.6437421440114921No Hit
ATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9550.42871251571197705TruSeq Adapter, Index 2 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8180.3672113485365416TruSeq Adapter, Index 2 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.2814688453941462No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.25004489136290176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.24106661878254623No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.17776979709103968No Hit
TATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3090.1387143113664931RNA PCR Primer, Index 2 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3030.13602082959238643No Hit
ACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2760.12390016160890645RNA PCR Primer, Index 2 (96% over 30bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.10055665289998203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)