FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379874
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91272.4026387696973206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54421.4325802766180364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38301.008229044367343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.6099390850650479No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA19700.518593007155004TruSeq Adapter, Index 2 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.33089919289027414No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.27061604637327114No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.23349847581040029No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.1737418196559912No Hit
ATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5320.1400464364499808TruSeq Adapter, Index 2 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4110.10819376951304906TruSeq Adapter, Index 2 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)