FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240933
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73643.0564513785990295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46321.922526179477282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30701.2742131629955216No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29901.241008911191078TruSeq Adapter, Index 2 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.8313514545537556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.4021864999813226No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.37271772650487894No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.3457392719137686No Hit
ATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8260.34283389988087976TruSeq Adapter, Index 2 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.2610684298124375No Hit
TTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5330.22122332764710523TruSeq Adapter, Index 2 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3450.14319333590666283No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.13779764498844077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3050.12659121000444107No Hit
ACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2870.11912025334844126RNA PCR Primer, Index 2 (96% over 30bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.11912025334844126No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.11123424354488591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT2520.10459339318399721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)