FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442201
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137963.119848213821317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80041.8100366123097869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64721.4635878254458945No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA51891.1734482735226741TruSeq Adapter, Index 2 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.7847110250768315No Hit
ATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA15260.34509193782917724TruSeq Adapter, Index 2 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA15160.3428305227713189TruSeq Adapter, Index 2 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.2539569109974876No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.20307507219567575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT7270.16440487470629872No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.15807291254429548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6790.15355008242857887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.15128866737072055Clontech SMART CDS Primer II A (100% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.12369940366484923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT5210.11781972451441763No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.11510602644498769No Hit
ACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA4970.11239232837555772RNA PCR Primer, Index 2 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA4680.10583422470776863RNA PCR Primer, Index 2 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)