FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002152938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773604
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142461.8415106436885023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81561.0542861722535044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68300.8828806469459827No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG42880.5542887575555452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.45604728000372285No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGG29580.38236617183985605No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT26760.34591341306404827No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24970.3227749597985533TruSeq Adapter, Index 2 (96% over 30bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT23020.2975682649003883No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA21140.2732664257165165No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCA20420.2639593383695017No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG18850.24366471734892786No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAG17430.2253090728589821No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC17050.220396998981391No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT16100.2081168142874132No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC14480.18717586775663No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA13860.17916143143003396No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT13580.17554200857286156No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC13280.17166405551160543No Hit
TGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCT13080.16907875347076798No Hit
GCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCA12430.16067652183804634No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12370.1599009312257951No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG11780.15227429020532468No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACC11710.1513694344910316No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA11480.14839633714406852No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC11420.1476207465318173No Hit
GAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGG11410.14749148142977545No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG11350.1467158908175242No Hit
GCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAA11350.1467158908175242No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT11340.14658662571548234No Hit
GTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT11330.14645736061344047No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCAC11320.14632809551139858No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG11310.14619883040935672No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC11230.14516470959302175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.14193308204197497No Hit
GGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTG10870.14051116591951437No Hit
GAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA10290.13301379000108582No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTC10150.13120407857249963No Hit
GCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGT9860.12745539061328534No Hit
GTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTAC9840.1271968604092016No Hit
GTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGC9740.12590420938878288No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA9740.12590420938878288No Hit
GTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTG8910.11517520591930756No Hit
GGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT8910.11517520591930756No Hit
TTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG8770.11336549449072135No Hit
CCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT8630.11155578306213515No Hit
GGGTTGGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAG8330.107677830000879No Hit
TCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTA8310.10741929979679525No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT8190.10586811857229281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.10548032326616719No Hit
GCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGTCTTTGGCGTCCGAGTTGTAATTT8030.10379987693962286No Hit
CGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATG7780.10056824938857607No Hit
GGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATT7740.10005118898040859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)