FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002152939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773604
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65490.8465571532722168No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG38450.49702431735099606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.4491962295955036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.3846929436766097No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGG26460.34203546000279217No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT23520.3040315200024819No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT20790.26874214714505096No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCA17450.22556760306306584No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG16780.21690684122626047No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAG16080.2078582840833295No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC15190.19635369000160288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.17709318979736402No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT13530.1748956830626522No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC13390.17308597163406603No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA13110.1694665487768936No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA12680.16390814938909312No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT12180.15744489428699954No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC11710.1513694344910316No Hit
CTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCT11610.15007678347061287No Hit
GAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGG11280.14581103510323112No Hit
GCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCA11230.14516470959302175No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACC10840.14012337061338878No Hit
TGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCT10790.1394770451031794No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG10670.13792586387867695No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG10600.13702100816438384No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG10550.1363746826541745No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCAC10460.13521129673579765No Hit
GCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAA10210.13197966918475085No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA10180.13159187387862523No Hit
GGGTACGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTG9910.1281017161234947No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC9840.1271968604092016No Hit
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAG9810.126809065103076No Hit
GTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT9570.12370670265407108No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT9510.12293111204181985No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT9150.11827756836831248No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA8420.10884121591925584No Hit
GTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGC8320.10754856489883713No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC8310.10741929979679525No Hit
TTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG8270.10690223938862778No Hit
GCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCCGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGT7980.10315355142941349No Hit
CCTTGCGGCTAGTACCCTTCTGCGCCAGAAGCTTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT7880.10186090040899477No Hit
GTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCGGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTAC7860.10160237020491104No Hit
CGTTGGGCCTTGGGGAGAAGCTTCTGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATG7780.10056824938857607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)