FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286025
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG71482.49908224805524Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG62562.1872213967310548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT28530.9974652565335198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21570.7541298837514203Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19070.6667249366314134Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.6223232234944498No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA17050.5961017393584477Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15810.5527488855869243Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14450.5052005943536404Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT13730.48002796958307836Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG12430.4345773970806747Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAGTGCCGTCCAGAATCCGAAGCTGCAAGCAATAGCCCGACGCACCCAACCCTTAGAGCCAATCCTTATCCC11770.4115024910409929No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10770.3765405121929901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.3674503976925094No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8540.2985752993619439Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8330.2912332838038633Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7890.2758500131107421Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7700.2692072371296215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7050.2464819508784197Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6730.2352941176470588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAATCGTACGCCAGACGCCCGAAGGCAGCTAAAGCCATTCACCTCAGTCTCCAGACAGC6560.22935058124289834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.20802377414561665No Hit
GTCCAACACCCTTCCACCAATAGCCCCACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA5880.20557643562625644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.2024298575299362No Hit
GTCCAGGCCAGTCCAAGTAGGTTGCCCCACCCAGTCCGACCCGGTCCAACACCCTTCCACCAATAGCCCCACGCA5710.19963289922209598No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT5600.19578708154881566No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5480.19159164408705534Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC5370.18774582641377502Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5160.18040381085569443Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCCAGACAGCTCCGCCCCAGTTGCCCAGAGTAGTGCCGTCCAGAATCCGAAGCTGCAAGCAATAGCCCGACG5120.17900533170177432No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5120.17900533170177432Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT5100.17830609212481427Clontech Universal Primer Mix Long (96% over 25bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGACGTGAAAGTTATGCGTTCCGTTGG5040.17620837339393408No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4920.17201293593217376No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGACGTGA4910.17166331614369373No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA4700.16432130058561314No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT4590.16047548291233282Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4580.16012586312385282Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGAGTAGTGCCGTCCAGAATCCGAAGCTGCAAGCAATAGCCCGACGCACCCAACCCTTAGAGCCAATCCTT4550.15907700375841272No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4390.15348308714273226Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAGAACGCACCGGACCCATCACAAGTGATGAGCTCTACCCACCATTCGACCTTAGCTCCGGATAATCCGATT4380.15313346735425223No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4320.15103574862337207No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4300.15033650904641205No Hit
GTTCTTGACAGTCCTTGAAAATCCAACGGAACGCATAACTTTCACGTCTGGTCGTACTCATAACCGCAGCAGGTC4100.14334411327681146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3980.13914867581505114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3900.1363517175072109Clontech SMART CDS Primer II A (96% over 26bp)
CCATATAACCTTAGCTCCGGATAATCCGATTTCAAGGTCGTAGGAGGTTAAGGAGAAAAGAGAACTCTTCCCAGG3820.13355475919937068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.13145704046849052No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3740.13075780089153047Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3710.1297089415260904Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3680.12866008216065028Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3560.12446464469888996No Hit
GTCAGGGACGCACCGGACACAACACAAGTGTTGTGCTCTACCCTCCATATAACCTTAGCTCCGGATAATCCGATT3560.12446464469888996No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTAATGCGTTCCGTGGG3550.12411502491040992No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.12341578533344988No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCAGACGCCCGAAGGCAGCTAAAGCCATTCACCTCAGTC3500.12236692596800978No Hit
GTTTTAAAGCGTGCACTTGTTTGCACCACTATCGAAAGGGAATCCGGTTAATATTCCGGAACCAGAATGTGGATC3440.12026920723712962No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA3430.1199195874486496No Hit
GTCCCTGACAGTCCTTGAAAATCCCACGGAACGCATTAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC3430.1199195874486496No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCAGACGCCCGAAGGCAGCTAAAGCCATTCACC3410.11922034787168953No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA3410.11922034787168953No Hit
GTTATATGGAGGGTAGAGCACAACACTTGTGTTGTGTCCGGTGCGTCCCTGACAGTCCTTGAAAATCCCACGGAA3380.11817148850624946No Hit
GTTCTAAGTCGGTTGTTAATTGTACGCGGGACGCCCGAAAGCTGCTAAAGGCGTCCGGTTGCCAGTCCAGGCCAG3370.11782186871776942No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCT3280.11467529062144917No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCGTGCACTTGTTTGCACCACTATCGAAAGGGAATCCGGTTAAT3250.1136264312560091No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3140.10978061358272878No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT3130.10943099379424875Clontech SMART CDS Primer II A (100% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3110.1087317542172887Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCACCCAGTCCGACCCGGTCCAACACCCTTCCACCAATAGCCCCACGCACCCAACCCTTAGAGCCAATCCTT3050.10663403548640853No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTGCGTCG2980.10418669696704833No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG2970.10383707717856831No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2960.10348745739008829Clontech Universal Primer Mix Long (96% over 25bp)
TGTTTGCACCACTATCGAAAGGGAATCCGGTTAATATTCCGGAACCAGAATGTGGATCTTTGACGGTAACGTAAA2900.10138973865920813No Hit
CTTGTGATGGGTCCGGTGCGTTCTTGACAGTCCTTGAAAATCCAACGGAACGCATAACTTTCACGTCTGGTCGTA2870.10034087929376803No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2870.10034087929376803Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)