FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225245
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61812.7441230659948057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38331.7017025905125531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27761.2324357921374505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.767608603964572No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA14960.664165686252747RNA PCR Primer, Index 24 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7660.3400741414903772No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.31299251925680927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.25527758662789407No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.2304157694954383No Hit
ATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4500.19978245910009101RNA PCR Primer, Index 24 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4210.1869075895136407Illumina PCR Primer Index 7 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2540.11276609913649582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10521876179271461No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.10388687873204733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT2340.10388687873204733No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)