FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207570
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50082.412680059738883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28731.3841113841113841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.960639784169196No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18190.8763308763308763RNA PCR Primer, Index 24 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.6643541937659585No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.2625620272679096No Hit
ATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5370.25870790576672925RNA PCR Primer, Index 24 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.23510141157199982No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.21823963000433588No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.19607843137254902No Hit
TTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3630.17488076311605724Illumina PCR Primer Index 7 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2770.13344895697836873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2400.11562364503540976No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10165245459363106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)