FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217227
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54372.502911700663361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33221.529275826669797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22391.0307190174333762No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17610.8106727064315209RNA PCR Primer, Index 24 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.6849977212777417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.36045242994655363No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.27989154202746436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.2775898023726332No Hit
ATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5490.2527310141004571RNA PCR Primer, Index 24 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.2476671868598287No Hit
TTATACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3190.14685098997822554Illumina PCR Primer Index 7 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2960.13626298756600239No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2820.12981811653247524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2570.11830941825831964No Hit
ACACATCTCCGAGCCCACGAGACGAACTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2260.10403863239836668RNA PCR Primer, Index 24 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)