FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160513
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGT3213220.018316273448256Illumina PCR Primer Index 4 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTT2025612.619538604349803Illumina PCR Primer Index 4 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCT146739.141315656675783Illumina PCR Primer Index 4 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTC96966.040632222935214Illumina PCR Primer Index 4 (95% over 22bp)
TCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTG71464.4519758524231685Illumina PCR Primer Index 4 (96% over 31bp)
TATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCT50643.1548846510874506Illumina PCR Primer Index 4 (95% over 23bp)
CATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCT35952.2396939811728642Illumina PCR Primer Index 4 (96% over 29bp)
CACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTG19021.1849507516525142Illumina PCR Primer Index 4 (96% over 27bp)
CTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGA13410.8354463501398641Illumina PCR Primer Index 4 (96% over 31bp)
CAGTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCC11590.7220598954601808No Hit
GCTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCG10680.6653666681203392No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAAGCTCGTATGCCGT10200.6354625482048183No Hit
CAGATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGT9930.6186414807523378Illumina PCR Primer Index 4 (95% over 21bp)
AGCTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCC7980.4971559935955343No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAACCTCGTATGCCGT7230.450430806227533No Hit
ATACACATCTCCGAGCCCACGAGACTCTGATCAAGCTCGTATGCCGTCTT6560.40868963884545173No Hit
ACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGC6400.3987215988736115Illumina PCR Primer Index 4 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTC6230.38813055640353117Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAAACTCGTATGCCGT6100.38003152392641093No Hit
CTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTC5850.3644564614704105Illumina PCR Primer Index 4 (95% over 22bp)
GACAGACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGT5750.3582264364880103Illumina PCR Primer Index 4 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTCTGATCAAGCTCGTATGCCGTCTTCT4780.29779519415872857RNA PCR Primer, Index 15 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCTGATCAACCTCGTATGCCGTCTT4420.27536710422208793No Hit
CAGTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCG4310.2685140767414477No Hit
ATACACATCTCCGAGCCCACGAGACTCTGATCAAACTCGTATGCCGTCTT4180.2604150442643275No Hit
GACAGATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCC4120.2566770292748874No Hit
GATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCT3890.242347971815367Illumina PCR Primer Index 4 (95% over 23bp)
CAGCTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGC3590.22365789686816642No Hit
ACACATCTCCGAGCCCACGAGACTCTGATCAAACTCGTATGCCGTCTTCT3250.20247581192800582RNA PCR Primer, Index 15 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCTGATCAACCTCGTATGCCGTCTTCT3060.1906387644614455RNA PCR Primer, Index 15 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTCTGATCAAGCTCGTATGCCGTC3050.19001576196320547No Hit
ATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTT3030.18876975696672543Illumina PCR Primer Index 4 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATATCGTATGCCGT2200.13706054961280395No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGATCAACCTCGTATGCCGTC2010.1252235021462436No Hit
TCTCCGAGCCCACGAGACTCTGATCAAGCTCGTATGCCGTCTTCTGCTTG1940.12086248465856349RNA PCR Primer, Index 15 (96% over 27bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCTGATC1820.11338645467968327No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGATCAAACTCGTATGCCGTC1730.1077794321955231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)