FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422589
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51391.2160751936278513No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG18170.42996859833076584No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8330.19711824018135826No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8230.1947518747530106No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8100.1916755996961587No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6740.1594930298706308No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTA5700.13488282941581536No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT5270.12470745807392052No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA5160.12210445610273811No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC5120.12115790993139906No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA4740.11216572130367804No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4710.11145581167517375No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4560.1079062635326523No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT4500.10648644427564372No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG4240.10033389416193984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)