FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282170
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT23160.8207817982067548No Hit
ATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGG19820.7024134387071623No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG19660.6967430981323316No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTC17900.6343693518091931No Hit
CCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAGGCAATTTG16860.597512138072793No Hit
GGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCC13880.4919020448665698No Hit
GGCCAGGGGGTAGCGACTGTTTAGCAAAAACACAGGGCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTG13690.4851685154339582No Hit
CTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGAAAGACCCCGT13340.472764645426516No Hit
CCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA11760.416770032250062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.379912818513662No Hit
GGGTGAGCCGGCCCCTAAGACGAGCCCGAAGGGGGTAGTCGATGGGAACCACGTTAATATTCGTGGGCCTGGTGG10620.37636885565439276No Hit
GAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAG10600.3756600630825389No Hit
CCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGA9890.3504979267817273No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTT9770.34624517135060423No Hit
GTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCAAGCTCTGAAATGAAGCCCAGGTAA9060.3210830350497927No Hit
GTGTAGCATAGGTGGGAGGCTTTGAAGCGTCGGCGCCAGCTGACGTGGAGCCATAGGTGAAATACCACCCTGTTG8260.2927313321756388No Hit
GTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGCAAGCTCTTGCCG8010.2838714250274657No Hit
GTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGAAAGACCCC7610.26969557359038876No Hit
CTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCA7360.2608356664422157No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGT7170.25410213700960416No Hit
GTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCCTCCCACCTATGCTACACAGTTCTTTCC6890.24417904100365026No Hit
GTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGC6680.23673671899918491No Hit
ACTTAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTA6680.23673671899918491No Hit
GTCTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCA6570.2328383598539887No Hit
CACCTATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAAC6470.2292943969947195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.22681362299323102No Hit
GGTCTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCC6170.21866250841691182No Hit
ACCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTG6120.21689052698727718No Hit
GTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGT5970.21157458269837331No Hit
TTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGA5940.21051139384059256No Hit
ATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCC5800.20554984583761562No Hit
CTTCAAAGCCTCCCACCTATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGT5210.18464046496792713No Hit
GCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGA5080.18003331325087715No Hit
GGTATACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGGGCTATTTCCCGGGACAGCTTC5010.17755253924938866No Hit
GTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT4840.17152780238863097No Hit
TGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGCAA4830.17117340610270404No Hit
GTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCA4730.16762944324343482No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTC4720.1672750469575079No Hit
GCATAAGCGCAAGCTCTTGCCGGGGGCACAGGCCAGGGGGTAGCGACTGTTTAGCAAAAACACAGGGCTCTGCTA4670.16550306552787325No Hit
GTCCCGGGAAATAGCCCCACCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCG4670.16550306552787325No Hit
GTCTGGTAATGGACCGTGGAGGTATCGGAAGTGCGAATGCAGACATGAGTAGCGATAAAGAGGGTGAGATGCCCT4630.16408548038416557No Hit
GAGCATGTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCG4600.1630222915263848No Hit
GTATCAACGCAGAGTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACG4480.15876953609526173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.15735195095155402No Hit
GGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAA4400.15593436580784634No Hit
GTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCG4360.15451678066413863No Hit
CCTTTACTGCAGCTTCAGAGTGGCATTAGGAAAGAACTGTGTAGCATAGGTGGGAGGCTTTGAAGCGTCGGCGCC4340.15380798809228477No Hit
CAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGAG4310.15274479923450401No Hit
GTTTAGCAAAAACACAGGGCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTG4280.15168161037672326No Hit
CTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCA4210.1492008363752348No Hit
AGGCTAATCCGCGCAGGGTGAGCCGGCCCCTAAGACGAGCCCGAAGGGGGTAGTCGATGGGAACCACGTTAATAT4060.14388489208633093No Hit
CAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTT4050.143530495800404No Hit
GGCTCACCCTGCGCGGATTAGCCTTGCGCAGGAACCCTTGGTCTTTCGGCGAGAGGGCATCTCACCCTCTTTATC4040.14317609951447707No Hit
CTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGC4000.14175851437076942No Hit
CTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCATC3970.14069532551298863No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCT3920.13892334408335402No Hit
GTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAG3800.13467058865223092No Hit
GTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGA3720.13183541836481555No Hit
TAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGC3640.12900024807740015No Hit
CTTCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACG3510.12439309636035015No Hit
CCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTTCAGGCACCGGGCAGGCGTCAGGCCCT3510.12439309636035015No Hit
TCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGA3500.12403870007442323No Hit
TCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCATCCGTCACACACCACCAGGC3400.12049473721515398No Hit
GGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGAGCATGTCCTGGAGACA3370.11943154835737321No Hit
CCTGTGAAGCGGTCTGGTAATGGACCGTGGAGGTATCGGAAGTGCGAATGCAGACATGAGTAGCGATAAAGAGGG3320.1176595669277386No Hit
GGCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTG3260.11553318921217706No Hit
CCCTAAGCTTCGGTGCGTGTCTTGAGCCCCGTTACATCTTCGCCGCAGGAACCCTTGTTTAGACCAGTGAGCTGT3240.11482439664032322No Hit
CAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAG3230.11447000035439629No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTT3220.11411560406846936No Hit
GCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGT3190.11305241521068858No Hit
ACGCAGAGTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCT3190.11305241521068858No Hit
CGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAGGCAATTTGCCGAGTTCCTTCAGGACACTTCTCTCAAGC3150.1116348300669809No Hit
GGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCCTCCCACCTATGCTACACAGTTC3100.10986284863734627No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTT3090.10950845235141937No Hit
TTCCACGAGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTCGCAGTCAGGCTGG3050.10809086720771166No Hit
GTAATGGACCGTGGAGGTATCGGAAGTGCGAATGCAGACATGAGTAGCGATAAAGAGGGTGAGATGCCCTCTCGC2950.10454690434844242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.1038381117765886No Hit
GCTTGCACCCCTCCACTTAACCTTCAGGCACCGGGCAGGCGTCAGGCCCTATACGTCGTCTTGAAGCCGACTTAG2910.10312931920473474No Hit
AGCTTAGGGTGTGTACTTTGTACACGCGGTAGCGGAGCGTTCCGTACGCCTGTGAAGCGGTCTGGTAATGGACCG2880.10206613034695396No Hit
ACACAGGGCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTG2830.10029414891731936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)