FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282170
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT20020.7095013644257008No Hit
ATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGG18210.6453556366729276No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTC17970.6368501258106815No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG16250.5758939646312506No Hit
CCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAGGCAATTTG14240.504660311159939No Hit
GGCCAGGGGGTAGCGACTGTTTAGCAAAAACACAGGGCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTG13160.46638551227983127No Hit
CTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGAAAGACCCCGT11980.42456675054045434No Hit
GGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCC11820.41889640996562355No Hit
GTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCAAGCTCTGAAATGAAGCCCAGGTAA10840.38416557394478507No Hit
CCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA10220.3621930042173158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.3423468122054081No Hit
GAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAG9270.32852535705425806No Hit
GGGTGAGCCGGCCCCTAAGACGAGCCCGAAGGGGGTAGTCGATGGGAACCACGTTAATATTCGTGGGCCTGGTGG8490.3008824467519581No Hit
CCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGA8460.2998192578941773No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTT8250.29237693588971186No Hit
GTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGAAAGACCCC8080.28635219902895415No Hit
CTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCA7610.26969557359038876No Hit
GTGTAGCATAGGTGGGAGGCTTTGAAGCGTCGGCGCCAGCTGACGTGGAGCCATAGGTGAAATACCACCCTGTTG7600.26934117730446183No Hit
GTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGCAAGCTCTTGCCG7270.2576460998688734No Hit
GGTCTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCC6620.23461034128362335No Hit
GTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCCTCCCACCTATGCTACACAGTTCTTTCC6510.2307119821384272No Hit
GTCTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCA6500.23035758585250027No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGT6100.21618173441542332No Hit
CACCTATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAAC6000.2126377715561541No Hit
GTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGC5930.21015699755466563No Hit
ACTTAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTA5840.20696743098132334No Hit
GTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGT5590.19810752383315022No Hit
TTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGA5470.19385476840202714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.19208278697239253No Hit
ATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCC5370.19031080554275792No Hit
ACCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTG5370.19031080554275792No Hit
GGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAA5280.1871212389694156No Hit
TGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGCAA4910.17400857639011943No Hit
GGTATACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGGGCTATTTCCCGGGACAGCTTC4640.1644398766700925No Hit
GTCCCGGGAAATAGCCCCACCGTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCG4570.16195910266860403No Hit
GTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT4410.15628876209377326No Hit
GCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGA4380.15522557323599248No Hit
GTATCAACGCAGAGTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACG4330.15345359180635787No Hit
GTCTGGTAATGGACCGTGGAGGTATCGGAAGTGCGAATGCAGACATGAGTAGCGATAAAGAGGGTGAGATGCCCT4210.1492008363752348No Hit
GTTTAGCAAAAACACAGGGCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTG4080.14459368465818478No Hit
GTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCA3940.13963213665520785No Hit
GTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCG3900.13821455151150017No Hit
CAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTT3890.13786015522557324No Hit
CTTCAAAGCCTCCCACCTATGCTACACAGTTCTTTCCTAATGCCACTCTGAAGCTGCAGTAAAGGTGCACGGGGT3870.13715136265371938No Hit
CAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGAG3860.13679696636779246No Hit
CTCTGAAGCTGCAGTAAAGGTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCA3800.13467058865223092No Hit
CCTTTACTGCAGCTTCAGAGTGGCATTAGGAAAGAACTGTGTAGCATAGGTGGGAGGCTTTGAAGCGTCGGCGCC3790.134316192366304No Hit
CTATACGGTGGGGCTATTTCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCATC3760.13325300350852323No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTC3760.13325300350852323No Hit
GGCTCACCCTGCGCGGATTAGCCTTGCGCAGGAACCCTTGGTCTTTCGGCGAGAGGGCATCTCACCCTCTTTATC3740.13254421093666938No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCT3690.13077222950703477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.1282914555055463No Hit
GCATAAGCGCAAGCTCTTGCCGGGGGCACAGGCCAGGGGGTAGCGACTGTTTAGCAAAAACACAGGGCTCTGCTA3580.12687387036183861No Hit
GTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGA3560.12616507778998476No Hit
TCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACGGA3560.12616507778998476No Hit
GAGCATGTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCG3450.1222667186447886No Hit
AGGCTAATCCGCGCAGGGTGAGCCGGCCCCTAAGACGAGCCCGAAGGGGGTAGTCGATGGGAACCACGTTAATAT3420.12120352978700784No Hit
GCTCTGCTAAGTCGGCTTCAAGACGACGTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGT3340.11836835949959246No Hit
CTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCGGCATAAGCGC3340.11836835949959246No Hit
GTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAG3320.1176595669277386No Hit
AGCTTAGGGTGTGTACTTTGTACACGCGGTAGCGGAGCGTTCCGTACGCCTGTGAAGCGGTCTGGTAATGGACCG3280.11624198178403089No Hit
TAGCAGAGCCCTGTGTTTTTGCTAAACAGTCGCTACCCCCTGGCCTGTGCCCCCGGCAAGAGCTTGCGCTTATGC3220.11411560406846936No Hit
CTTCCCCACTGTCTCCAGGACATGCTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCCGCGGTTAGACG3170.11234362263883475No Hit
ACGCAGAGTACATGGGCGCAGGTTAGACATCAGAAAACAACAGGGTGGTATTTCACCTATGGCTCCACGTCAGCT3120.11057164120920013No Hit
CGCTTATGCCGGGCCTCCTTCTTCCGAAGGTACGGAGGCAATTTGCCGAGTTCCTTCAGGACACTTCTCTCAAGC3050.10809086720771166No Hit
TCCCGGGACAGCTTCGAAGCCGGATCAATCCGATAAGATCCGACAACACACGCCATCCGTCACACACCACCAGGC3040.10773647092178473No Hit
GGGTGGTATTTCACCTATGGCTCCACGTCAGCTGGCGCCGACGCTTCAAAGCCTCCCACCTATGCTACACAGTTC3030.1073820746358578No Hit
CAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAG2960.10490130063436935No Hit
GGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGAATTCAATTTCGCTGAGCATGTCCTGGAGACA2940.1041925080625155No Hit
TGTCTAACCTGCGCCCATGAAACTGGGCGAGGGACCCTCTGTGGCGGGTAGTTTGACTGGGGCGGTCGCCTCCTA2860.10135733777510011No Hit
GCATAGGTGGGAGGCTTTGAAGCGTCGGCGCCAGCTGACGTGGAGCCATAGGTGAAATACCACCCTGTTGTTTTC2830.10029414891731936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)