FastQCFastQC Report
Wed 8 May 2024
EGAF00002153295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386045
Total Bases28.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGA53831.394397026253416No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT46421.202450491523009No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCT46171.1959745625509979No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGC43831.1353598673729748No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA43181.118522452045746No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGC35620.9226903599321323No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGG33840.8765817456514138No Hit
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACG31020.8035332668471291No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAAC31000.8030151925293683No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCA30540.791099483220868No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA28580.7403282000803015No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC27800.7201233016876271No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG27170.7038039606781593No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT26690.6913701770518981No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTT26110.6763460218368325No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCAC25120.6507013431076688No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA23390.6058879146213525No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGT23000.5957854654250152No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGG22020.570399823854732No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCG20520.5315442500226658No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCC20160.5222189123029699No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA19210.49761038220932796No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA18520.4797368182465775No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATT17390.45046561929308765No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA16450.42611612635832613No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG16080.4165317514797498No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAA15720.4072064137600539No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA15500.40150759626468413No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGC15340.39736300172259714No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC15040.38959188695618385No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTC14700.38078462355424886No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT14670.3800075120776075No Hit
CTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAA14570.37741714048880315No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGG14430.37379062026447696No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGT14360.37197736015231386No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT14290.37016410004015077No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTA14290.37016410004015077No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT13700.3548809076662047No Hit
GGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGG13380.3465917185820306No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT12710.329236228937041No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAA12700.3289771917781606No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTT12470.3230193371239104No Hit
GGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCAC12190.3157662966752581No Hit
TCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTG12020.31136266497429055No Hit
GCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGC11800.30566384747892084No Hit
GCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAG11410.29556139828258365No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA10910.28260954033856156No Hit
CTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTA10530.2727661283011048No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCG10330.267585385123496No Hit
TGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAA10180.2636998277402893No Hit
GGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCC10070.26085041899260447No Hit
GCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCAC10020.2595552331982023No Hit
GCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTT9990.25877812172156095No Hit
GCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAG9960.25800101024491967No Hit
GGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCA9560.247639523889702No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGA9560.247639523889702No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG9540.2471214495719411No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA9440.24453107798313667No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG8930.23132018288023418No Hit
TGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCA8900.23054307140359281No Hit
GTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCT8870.2297659599269515No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTC8720.22588040254374492No Hit
GATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGG8670.2245852167493427No Hit
GAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTT8630.22354906811382094No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTC8590.22251291947829915No Hit
GGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCC8560.22173580800165785No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCG8540.22121773368389697No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGC8410.2178502506184512No Hit
CTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCT8250.21370565607636416No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTT7900.2046393555155487No Hit
GCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACC7850.20334416972114652No Hit
CCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCC7770.20127187245010297No Hit
TACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGC7740.20049476097346164No Hit
GGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACT7710.19971764949682033No Hit
GGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAA7500.19427786916033105No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAA7280.18857905166496133No Hit
GTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTCACGG7180.18598868007615693No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTC6920.17925371394526546No Hit
GTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGT6820.17666334235646103No Hit
CCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGG6730.17433200792653705No Hit
GCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA6730.17433200792653705No Hit
GGCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAG6690.1732958592910153No Hit
CCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCT6470.16759704179564558No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGG6430.16656089316012382No Hit
AGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTT6360.16474763304796075No Hit
GTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAAT6320.16371148441243896No Hit
GCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT6260.16215726145915632No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT6250.16189822430027587No Hit
GTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAAC6160.15956688987035192No Hit
GGATTATCCGCGGCAACGCAACTGAAGGTGGAGACGTCGGCGGGGGCCCC6070.15723555544042794No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAAC6050.15671748112266704No Hit
GAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCCGGT5980.15490422101050394No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA5840.15127770078617778No Hit
GGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAACTGGTACGG5720.1481692548796125No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGC5640.14609695760856894No Hit
GCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAA5580.1445427346552863No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTC5520.14298851170200366No Hit
GTATCAACGCAGAGTACATGGGGGAAAAGGATTGGCTCTAAGGGTTGGGT5490.14221140022536233No Hit
CTTCTTAACTGTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTT5460.14143428874872102No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGG5420.14039814011319923No Hit
GAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTT5410.14013910295431878No Hit
CACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTG5410.14013910295431878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.13936199147767747No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT5340.1383258428421557No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGC5310.13754873136551438No Hit
GTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAA5300.13728969420663395No Hit
GTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGA5300.13728969420663395No Hit
CTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCT5250.13599450841223173No Hit
ACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAG5170.1339222111411882No Hit
AGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACGCTCTTGGCAG5050.1308137652346229No Hit
GTACATGGGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCTTG4990.12925954228134026No Hit
CTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATATTCCGGTACCTCG4970.12874146796357938No Hit
CTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTC4910.12718724501029674No Hit
CATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4890.12666917069253583No Hit
GGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGAT4840.12537398489813364No Hit
GAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGA4780.123819761944851No Hit
GTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAA4780.123819761944851No Hit
CCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTG4760.12330168762709011No Hit
CGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGT4750.12304265046820967No Hit
GTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT4690.12148842751492701No Hit
GTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATT4570.11837998160836173No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAA4560.11812094444948128No Hit
ACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTA4540.1176028701317204No Hit
GCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGA4540.1176028701317204No Hit
CCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTC4480.11604864717843774No Hit
GCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAA4440.115012498542916No Hit
GGTTAATATTCCGGTACCTCGATGTGGATTATCCGCGGCAACGCAACTGA4440.115012498542916No Hit
GCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGAT4420.1144944242251551No Hit
CTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCG4370.1131992384307529No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGAC4340.11242212695411156No Hit
CCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCC4330.11216308979523112No Hit
ACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGAT4290.11112694115970936No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTC4290.11112694115970936No Hit
GGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCG4270.11060886684194848No Hit
GCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGC4260.11034982968306803No Hit
GCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATG4250.11009079252418759No Hit
CTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCC4130.10698234661762229No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC4090.10594619798210055No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGA4050.10491004934657876No Hit
ATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG4050.10491004934657876No Hit
CTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGT4050.10491004934657876No Hit
CACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATT4010.10387390071105701No Hit
GTATCAACGCAGAGTACATGGGGAAAAGGATTGGCTCTAAGGGTTGGGTG3950.10231967775777435No Hit
GCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAA3930.10180160344001347No Hit
ATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTT3910.10128352912225258No Hit
AGCCCTGGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCAC3890.10076545480449171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)