FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236832
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36661.5479327117957031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.8546142413187408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.5324449398729901No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.4623530603972436No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.23012092960410757No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.22378732603702203No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.1541176867990812No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC3450.14567288204296716No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC3300.13933927847588162No Hit
CCTATGTATTCAGCATGCAATGACCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTTG3020.127516551817322No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2920.12329414943926496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.12244966896365356No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2730.11527158492095663Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA2610.11020470206728822No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA2500.10556005945142548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)