FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198173
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG8460.4268997290246401No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATGA7220.3643281375363949No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGG6990.35272211653454305No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGA6400.322950149616749No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAAT6050.3052888133095831No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGA5650.2851044289585362No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAG5310.26794770226014647No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5300.2674430926513703No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT5240.26441543499871323No Hit
GTCTAGATGAACCAACACCTTTTGTGGTGTCTGATGAGCGTGTATTCCGGCACTTTAACCCCCCGTTCGGTTCAT5150.25987394851972767No Hit
GCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC5150.25987394851972767No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG5040.25432324282318985No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG4940.24927714673542817No Hit
ATCCTGATGTCTTCGGAAGGATTTGAGTAAGAGCATGGCTGTTGGGACCCGAAAGATGGTGAACTATGCTTGAAT4920.2482679275178758No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAAT4880.2462494890827711No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC4840.24423105064766645No Hit
CATCAGGATCGGTCGATAGTGCACCCTTGCGGGTTCCTACCTCCGTTCACTTTCATTACGCGCTCGGGCTTGACA4830.24372644103889027No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCA4770.2406987833862332No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4640.23413885847214302No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT4570.23060659121070987No Hit
CATCAGACACCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA4470.22556049512294815No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGGCGGGGGGGTAT4460.22505588551417197No Hit
ACTTATACCCCCCCGCCGGGGCAAAATTTACGCCCCGGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG4430.22354205668784344No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGT4370.22051439903518644No Hit
GCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA4340.2190005702088579No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAG4290.21647752216497704No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTC4180.2109268164684392No Hit
GAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA4040.2038622819455728No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4040.2038622819455728No Hit
ACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA4020.20285306272802045No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAA3980.2008346242929158No Hit
CCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC3940.1988161858578111No Hit
GCACTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGG3910.1973023570314826No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGG3850.19427469937882558No Hit
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCC3820.19276087055249705No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGC3790.19124704172616855No Hit
GGTATAAGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACATTCCT3690.18620094563840686No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT3580.18065023994186896No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG3580.18065023994186896No Hit
GGATATGGATTCTCCGCGGCAACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCT3560.17964102072431662No Hit
CTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC3540.1786318015067643No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3370.17005343815756938No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTATACCCCCCCG3370.17005343815756938No Hit
GCCTTATAGGGGAGACGACATATTACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCT3360.16954882854879325No Hit
GGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGGGGGCTGAAACAGCCTTCACC3350.16904421894001706No Hit
ATTCAAGCATAGTTCACCATCTTTCGGGTCCCAACAGCCATGCTCTTACTCAAATCCTTCCGAAGACATCAGGAT3330.16803499972246472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3310.16702578050491237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAATGTGTAAC3300.16652117089613622No Hit
TCCATATCCAGATACCAGAATTTTAACTGGTTTCCCTTTCGAGGGCGCGGCGCAAGCGCCGACATTAAAACGGAA3290.16601656128736003No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAAGCATAGTTCACCATCTTTCG3250.16399812285225535No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGCATAGGGGCGAAAGACTAATCGAACTATCTAGTAGCTGGTTC3210.16197968441715066No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3180.16046585559082216No Hit
GGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAATTCTGGTATCTGGA3170.159961245982046No Hit
GTTGAATGAAACGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGGGGGGTT3170.159961245982046No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGC3120.15743819793816513No Hit
TGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCT3030.1528967114591796No Hit
CTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATCCATATCCAGATACCAGAATT3000.1513828826328511No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA3000.1513828826328511No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATGAACTAGCCC2980.15037366341529876No Hit
TGCTTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGTCAA2950.14885983458897023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2940.14835522498019407Clontech Universal Primer Mix Long (96% over 26bp)
GCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGG2910.14684139615386554No Hit
GCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAA2860.1443183481099847No Hit
CCTCCACGCCTGCCTACTCGCCGGGGCGTAAATTTTGCCCCGGCGGGGGGGTATAAGTAACACGCTTGAGCGCCA2840.14330912889243239No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT2840.14330912889243239No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCG2830.1428045192836562No Hit
CTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCCGGGGCGT2830.1428045192836562No Hit
TCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA2780.14028147123977536No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTA2650.13372154632568514No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATGTACCTCCCATTTAGAGCTGCATT2640.13321693671690896No Hit
TCATTCAACTGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATATTTAAAGTTTGAGAATAGGTGAAGGCTG2600.1311984982818043No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGG2590.1306938886730281No Hit
GTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGGAGGTAGGAACCCGCAA2550.12867545023792343No Hit
GGCGTGGAGGTCCGTGACGAAGCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGT2540.12817084062914727No Hit
GCTAAGGAATGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTA2530.12766623102037108No Hit
CGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGGGGGGTTAAAGTGCCGGA2490.1256477925852664No Hit
CTTCTGCAGGCAGGCCAGCATCAGTTTGGGCGGTAAGATAAAGGTCTCTGTCACGTACCTCTCTTCGGGGAGGCC2480.12514318297649024No Hit
GAGGTAGGAACCCGCAAGGGTGCACTATCGACCGATCCTGATGTCTTCGGAAGGATTTGAGTAAGAGCATGGCTG2470.12463857336771408No Hit
GTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGC2440.12312474454138556No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC2440.12312474454138556No Hit
CACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTC2440.12312474454138556No Hit
CTAATAACGTTTCATTCAACTGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA2440.12312474454138556No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG2430.12262013493260937No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2420.12211552532383323No Hit
ATCTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTC2380.12009708688872853No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT2370.11959247727995237No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAATC2360.1190878676711762No Hit
CCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCA2350.11858325806240003No Hit
GCGCTCAAGCGTGTTACTTATACCCCCCCGCCGGGGCAAAATTTACGCCCCGGCGAGTAGGCAGGCGTGGAGGTC2350.11858325806240003No Hit
GTGCACTCTTCTGCAGGCAGGCCAGCATCAGTTTGGGCGGTAAGATAAAGGTCTCTGTCACGTACCTCTCTTCGG2340.11807864845362384No Hit
ACGCAACTGAGAGCGGAGACCTTGGCGGGAGCCCCAAGAAGAGTTCTCTTTTCTTCTTAACAGTCTGTCACCCTG2340.11807864845362384No Hit
GGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTT2320.11706942923607151No Hit
CTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACATTCCTTAGCGGATTCCGACT2290.115555600409743No Hit
GTGCCCTTCTGCGCCAGAGGCGTCTCCCCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2230.11252794275708598No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACT2210.11151872353953364No Hit
GGGTTAAAGTGCCGGAATACACGCTCATCAGACACCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGC2210.11151872353953364No Hit
GCGTGGAGGTCCGTGACGAAGCCTTGGGGGTGACCCCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTA2200.11101411393075747No Hit
CAGTTGCGTTGCCGCGGAGAATCCATATCCAGATACCAGAATTTTAACTGGTTTCCCTTTCGAGGGCGCGGCGCA2200.11101411393075747No Hit
GAATTACCCTATCTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTC2180.11000489471320514No Hit
CTAAGAGATAGGGTAATTCCGTTTTAATGTCGGCGCTTGCGCCGCGCCCTCGAAAGGGAAACCAGTTAAAATTCT2180.11000489471320514No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCGTGTGA2140.10798645627810045No Hit
GATTAGAGGCCTGGGGGCTGAAACAGCCTTCACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTA2110.10647262745177193No Hit
GCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGGGGGCTGAAACAGCCTTCACCTATTC2110.10647262745177193No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCATGAAAACTATTTCTTCAA2110.10647262745177193No Hit
CTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAGGTCTCCGCTCTCAGTTGCGTTGCCGCGGAGAA2080.10495879862544343No Hit
AACGTGGACAGTTGAATGAAACGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGA2070.10445418901666725No Hit
CGTTACTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGC2070.10445418901666725No Hit
TCCTACCTCCGTTCACTTTCATTACGCGCTCGGGCTTGACACCCAAACACTCGCATAGATGTTAGACTCCTTGGT2070.10445418901666725No Hit
CTATAAGGCCTCCCCGAAGAGAGGTACGTGACAGAGACCTTTATCTTACCGCCCAAACTGATGCTGGCCTGCCTG2040.10294036019033875No Hit
TTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACCAACACCTTTTGTGGTGTCTGAT2030.10243575058156258No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA2000.10092192175523407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)