FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273997
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG87853.206239484373916Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG78372.860250294711256Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT42401.5474621984912242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28681.0467267889794414Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24920.9094990091132386Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA23640.8627831691587864Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21450.7828552867367161Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20470.7470884717715888Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.7138764293039705No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG18330.6689854268477392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC18280.6671605893495185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT17970.6558465968605496Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15520.5664295594477312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCG14720.5372321594761986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.42774190958295166No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11660.42555210458508674Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG11480.4189826895914918Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10730.3916101271181801Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGG10130.36971207713953075No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9300.3394197746690657Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9290.3390548071694216Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAG8800.3211713996868579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.300368252207141No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8130.2967185772106994Clontech Universal Primer Mix Long (96% over 26bp)
GATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTGGGTGGCCAAG7600.2773752997295591No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7550.2755504622313383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCGGATCCTCCCCTCGGACCTTGAAAATCCGGGCGAGGGCCACGAGGAACACCGCGCCGGCTCGTACCCATAT7510.27409059223276167No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7480.2729956897338292Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC7260.2649664047416578Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTA7210.26314156724343696No Hit
TTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGG7020.256207184750198No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6630.2419734522640759Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.2343091347715486No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG6150.22445501228115636Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5810.21204611729325504Clontech Universal Primer Mix Long (96% over 26bp)
GAATAATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCTCTGAGGATCGTGGC5750.2098563122953901No Hit
GTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGG5580.20365186480143946No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5580.20365186480143946Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGA5500.20073212480428618No Hit
GTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACC5240.1912429698135381No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5120.18686335981780824Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCT4980.18175381482279002No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.17992897732456925No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA4900.17883407482563676Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT4860.17737420482706015Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4780.1744544648299069Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4720.17226465983204198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT4550.1660602123380913Clontech SMART CDS Primer II A (100% over 26bp)
GCTCTGAGGATCGTGGCGCGCCGGGCTTGGTCGGGAAGTGGAGCGGCGCCAACGTGCCGGGCCTGGGCGAGGCGA4410.1609506673430731No Hit
CCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGGAA4360.15912582984485232No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4300.15693602484698738Clontech Universal Primer Mix Long (96% over 26bp)
TAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCG4290.15657105734734322No Hit
CAGCAAGGAAGATCCGTGGGTGGCCAAGGCACGGGACCGAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCG4250.15511118734876658No Hit
GCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTG4150.15146151235232502No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAA4030.14708190235659516No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4000.14598699985766267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.14525706485837436No Hit
GGCCTGGGCGAGGCGAAGAGCGCTTCGTCGCTCGGACCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTT3920.14306725986050942No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3900.1423373248612211Clontech SMART CDS Primer II A (96% over 26bp)
CTCTATAAGCGTTCGAGTTCCCTGGAAGCCTCTAGGAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCACTTA3710.13540294236798214No Hit
GGATTGGCTCTGAGGATCGTGGCGCGCCGGGCTTGGTCGGGAAGTGGAGCGGCGCCAACGTGCCGGGCCTGGGCG3700.13503797486833796No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAATAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAA3660.13357810486976135No Hit
GAGGAACACCGCGCCGGCTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAATAGCCTCTAGTCGATAGAATAA3620.1321182348711847No Hit
GAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCGCCCAGGCCCGGCACGTTGGCGCCGCTCCACTTCCCGAC3610.13175326737154056No Hit
GTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCG3610.13175326737154056No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCG3610.13175326737154056No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC3600.1313882998718964Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT3520.12846855987474315Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3510.128103592375099Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTC3490.1273736573758107No Hit
GCACGGGACCGAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCGCCCAGGCCCGGCACGTTGGCGCCGCTCC3440.1255488198775899No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT3370.1229940473800808Clontech Universal Primer Mix Long (96% over 26bp)
GTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCAC3340.12189914488114834No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCTCTGAGGATCGTGGCGCGCC3310.12080424238221586No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT3280.11970933988328339Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGTAGGGAGATCCGGGGAGAGTTGTCTCTTCTCTATAAGCGTTCGAGTTCCCTGGAAGCCTCTAGGAGGGAGA3220.11751953488541844No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3160.11532972988755352Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCAAGGTGAATAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCG3160.11532972988755352No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3140.1145997948882652Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAGAGCCAATCCTTATCCCGAAGTTACAGATCGGTTTTGCCGACTTCCCTTACCTACATTATTCTATCGACT3130.11423482738862105No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC3120.11386985988897688Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3110.11350489238933273Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT3090.11277495739004442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGA3060.11168005489111195No Hit
GTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGGAAACGGTGGACG3040.11095011989182364No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT3020.11022018489253531Clontech Universal Primer Mix Long (96% over 26bp)
ATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTGGGTGGCCAAGG2920.10657050989609375No Hit
GACCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGG2890.10547560739716129No Hit
GTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTGGGTGGCCAAGGCACGGGACC2860.10438070489822882No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC2860.10438070489822882Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTT2830.10328580239929634No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2830.10328580239929634Clontech Universal Primer Mix Long (96% over 25bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2810.10255586740000804Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2810.10255586740000804Clontech Universal Primer Mix Long (96% over 25bp)
GCCCAGGCCCGGCACGTTGGCGCCGCTCCACTTCCCGACCAAGCCCGGCGCGCCACGATCCTCAGAGCCAATCCT2780.10146096490107556No Hit
GTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGA2750.10036606240214309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)