FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273997
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG64542.3555002427033873Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG59122.157687857896254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31911.164611291364504Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22210.810592816709672Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20470.7470884717715888Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA18170.6631459468534326Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.6379631893779858No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16360.5970868294178404Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14220.5189837844939909Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG14030.5120494020007518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13490.4923411570199674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13310.4857717420263726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT13180.4810271645309985Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCG13120.47883735953313356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.3576681496512735No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG9480.34598918966266057Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9190.33540513217298Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGG9040.32993061967831766No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8480.30949243969824486Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7680.2802950397267123Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAG7660.279565104727424No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7390.26971098223703177Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT7270.26533137224130193Clontech SMART CDS Primer II A (96% over 26bp)
GATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTGGGTGGCCAAG6450.23540403727048106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.23175436227403948No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6190.22591488227973297Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTA5820.2124110847928992No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5720.20876140979645763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5600.20438179980072774Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5400.19708244980784462Clontech Universal Primer Mix Long (96% over 26bp)
TTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGG5100.1861334248185199No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5060.1846735548199433Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT4960.1810238798235017Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4900.17883407482563676Clontech Universal Primer Mix Long (96% over 26bp)
GAATAATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCTCTGAGGATCGTGGC4810.17554936732883936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.1737245298306186No Hit
GTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACC4730.1726296273316861No Hit
GTCCGGATCCTCCCCTCGGACCTTGAAAATCCGGGCGAGGGCCACGAGGAACACCGCGCCGGCTCGTACCCATAT4650.16970988733453285No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGA4570.16679014733737962No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCT4550.1660602123380913No Hit
GTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGG4340.15839589484556402No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG4190.15292138235090166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT4020.14671693485695098Clontech Universal Primer Mix Long (96% over 25bp)
GCTCTGAGGATCGTGGCGCGCCGGGCTTGGTCGGGAAGTGGAGCGGCGCCAACGTGCCGGGCCTGGGCGAGGCGA4020.14671693485695098No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3990.14562203235801852Clontech Universal Primer Mix Long (96% over 26bp)
CAGCAAGGAAGATCCGTGGGTGGCCAAGGCACGGGACCGAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCG3980.14525706485837436No Hit
CCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGGAA3730.13613287736727045No Hit
TAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCG3720.13576790986762627No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3670.1339430723694055Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3620.1321182348711847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3610.13175326737154056Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCG3560.1299284298733198No Hit
GGCCTGGGCGAGGCGAAGAGCGCTTCGTCGCTCGGACCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTT3420.12481888487830158No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCG3420.12481888487830158No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3310.12080424238221586Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTC3310.12080424238221586No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.1204392748825717No Hit
GGATTGGCTCTGAGGATCGTGGCGCGCCGGGCTTGGTCGGGAAGTGGAGCGGCGCCAACGTGCCGGGCCTGGGCG3290.12007430738292756No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTTGGGGCCATCGCAATGCTTTGTTTTAA3250.11861443738435092No Hit
ATGTAGGTAAGGGAAGTCGGCAAAACCGATCTGTAACTTCGGGATAAGGATTGGCTCTGAGGATCGTGGCGCGCC3160.11532972988755352No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT3160.11532972988755352Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3140.1145997948882652Clontech Universal Primer Mix Long (96% over 26bp)
GAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCGCCCAGGCCCGGCACGTTGGCGCCGCTCCACTTCCCGAC3120.11386985988897688No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3070.11204502239075609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGAAGAGCGATCCGGACCGTCCACCGTTTCCGGCGACCAGACACCCGGAGGCTCGCAGCAAGGAAGATCCGTG3050.11131508739146778No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAATAGCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAA3030.11058515239217948No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.10985521739289117No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3000.109490249893247Clontech SMART CDS Primer II A (96% over 26bp)
CCTCAGAGCCAATCCTTATCCCGAAGTTACAGATCGGTTTTGCCGACTTCCCTTACCTACATTATTCTATCGACT2980.1087603148939587No Hit
GTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGGAAACGGTGGACG2970.10839534739431453No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2910.1062055423964496Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT2910.1062055423964496Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT2880.10511063989751712Clontech Universal Primer Mix Long (96% over 26bp)
GCACGGGACCGAGCTAGGGTCCGAGCGACGAAGCGCTCTTCGCCTCGCCCAGGCCCGGCACGTTGGCGCCGCTCC2840.10365076989894051No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC2830.10328580239929634Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT2830.10328580239929634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGTGTCTGGTCGCCGG2810.10255586740000804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)