FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179845
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7530.4186938752814924Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG7000.3892240540465401Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3910.2174094359031388Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCTGGACGGGTCCATCCTGGCGAACCAAGACAGTCCCATCCTGGTCCCGCGGCTTCCAACCAAGCCCCGACGT3570.19850426756373546No Hit
AACCAAGACAGTCCCATCCTGGTCCCGCGGCTTCCAACCAAGCCCCGACGTACCCAAGCCTTAGAGCCAATCCTT3380.1879396146681865No Hit
GTCCCATCCTGGTCCCGCGGCTTCCAACCAAGCCCCGACGTACCCAAGCCTTAGAGCCAATCCTTTTCCCGAAGT2850.15846979343323417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2400.13344824710167089Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGA2320.12899997219828185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.1278879034724346No Hit
GTTCTAAGTTGCTTGTTAAGCGCACGCCGGATGCCCGAAAGCAGACAAGGCCATCCACACGCAGGTCCTGGACGG2290.12733186910951097No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2250.12510773165781644Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGCTTGGGTACGTCG2240.12455169729489281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2160.12010342239150379Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT2060.1145430787622675No Hit
GTATGGAGCTAGGGTTCAATGGCGGGTAGAGCTCGGCACTTTTGCCGGGTCCGGTGCGCTCTTGACAGTCCTTGA2040.11343101003642025No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1970.10953876949595484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCCTGGACGGGTCCATCCTGGCGAACCAAGACAGTCCCATCCTGGTCCCGCGGCTTCCAACCAAGCCCCGACG1950.1084267007701076No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1950.1084267007701076Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)