FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513918
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60091.169252682334536No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG52371.0190341649835188Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG48620.9460653255966905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.7423363260286661No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT27160.5284889807323347Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.4677789063624936No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18320.35647710335111826Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA17440.3393537490416759Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17230.3352674940360135Clontech Universal Primer Mix Long (96% over 26bp)
ACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGG15800.30744204328316965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.30277203756241267No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC15500.30160453613222343Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15240.29654536326807Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAG14760.287205351826556No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT14540.2829245132491954No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11570.22513319245482744Clontech Universal Primer Mix Long (96% over 26bp)
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG11570.22513319245482744No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11180.2175444331585973Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11030.21462567958312417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10120.19691857455858716Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10090.19633482384349257Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9500.18485439311329824Clontech Universal Primer Mix Long (96% over 26bp)
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCAAGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC9480.1844652259699018No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT9320.18135188882273048No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCTTAG9250.179989803820843No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGATGCCTCTAATCATTGGCTTTACCCG8840.17201187738121645No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG8840.17201187738121645No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCACGTGCGAGTTCTATCGGGTAAAGCC8800.17123354309442362No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC8370.16286644951140067No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA7980.15527769021517052No Hit
GCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGGCGCGGCGGTC7890.15352643806988664No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGAC7780.15138601878120633No Hit
CTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCC7530.1465214294887511No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.13757058519063353No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG6950.135235582330255No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT6820.1327059958981783No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.12900890803591233No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCG6580.12803599017742132No Hit
CTCCTACTCATCGGGGCCTAGAACTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCG6460.1257009873170428No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.12375515160006072No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6310.12278223374156967Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGC6170.12005806373779475No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGATCGTTCCCAGTCCGTCCCCCGGCCGGCACG6100.11869597873590729No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCT6060.11791764444911446No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6040.11752847730571803Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGC5870.11422055658684847No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGG5770.1122747208698664No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGG5700.11091263586797895No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG5670.1103288851528843Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGC5540.1077992987208076No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGAC5540.1077992987208076No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCC5480.10663179729061836No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAGGCGATCGTTCCCAGTCCG5360.10429679443023986No Hit
TGCCAGGAGCGCGCAGATGCCGAGGCACGCCGTGAGGCGCGCGCTGCCAACCACGATCGCGGCAACGACGTCTCC5280.10274012585665417No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA5220.10157262442646493No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCT5200.1011834572830685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)