FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002153371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820781
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTTAGCCATTAAGGCAAATGCGAGGCCTTAATGAGTAGGAGGGCGTGGAGGTCGTGGCGAAGCCTGGG58470.7123702912226282No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTC30340.3696479328834366No Hit
ATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCCAGGG29970.36514003126290695No Hit
GCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTTCAACCCGTCAGCACGTGGCAGTCCGCCCTCGCCGCGT27500.33504674206639773No Hit
GCCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT25320.3084866730589524No Hit
AGTATAGGTCGCGCGCTTTAGCGCCATCCATTTTCGGGGCTAATTGATTCGGCAGGTGAGTTGTTACACACTCCT24340.2965468255234953No Hit
GATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA24200.29484113301843No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCTTTGGGACCATCGCAATGCTTTGTTTTA24190.2947192978394968No Hit
TACCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGAGCGCGA24110.2937446164080309No Hit
GTTCTGAGTCGGTTGTTCACTGCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTTCAACCCGTCAGCACGT23480.28606900013523706No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGGTTGTTCACTGCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTT22420.27315447116831404No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22110.2693775806213838Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG19970.2433048523296714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTAAGAGATGGGGTAATCCTGTATCAAGGGCGCAATTTGCGCCGCCCATCGAAAGGGAATCGGGTTAATATT19690.2398934673195408No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGAGCGCGATCGGTATCCGCTCCTCCGA19630.23916245624594137No Hit
GTATAGGTCGCGCGCTTTAGCGCCATCCATTTTCGGGGCTAATTGATTCGGCAGGTGAGTTGTTACACACTCCTT19170.23355803801501251No Hit
CCCTTCAACCCGTCAGCACGTGGCAGTCCGCCCTCGCCGCGTGAGCAGCTCGGGCAGACCACCAACAGCTTGGGG18970.2311213344363478No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCTTTGGGACCATCGCAATGCTTTGTTTTAATTAAA18580.22636976245795165No Hit
GAGTACGACCGAGCGCGATCGGTATCCGCTCCTCCGAATTTTCAAGGGCCGTCAGGGGCGCACCAGACACCGCGA17970.21893781654302424No Hit
CCTTTGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGGT17890.21796313511155838No Hit
GTATCCGCTCCTCCGAATTTTCAAGGGCCGTCAGGGGCGCACCAGACACCGCGAGACGTGCGATGCTTTCCCAGT17830.21723212403795897No Hit
GTCTGGTGCGCCCCTGACGGCCCTTGAAAATTCGGAGGAGCGGATACCGATCGCGCTCGGTCGTACTCATAACCG17620.214673585280361No Hit
ATCCTGTATCAAGGGCGCAATTTGCGCCGCCCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACGCGGC17580.21418624456462806No Hit
AGCGGATACCGATCGCGCTCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGA16900.2059014523971681No Hit
GTGCCCAACTGCGCGCTAACCTAGATCCCACAAAGGGTGTTGATTGATATAGACAGCAGGACGGTGGTCATGGAA16260.19810400094544098No Hit
GTCCGTACCAGTTCTGAGTCGGTTGTTCACTGCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTTCAACCC16180.1971293195139751No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGGTTGTTCACTGCCC14640.17836670195825682No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA14630.17824486677932358No Hit
GTCTATATCAATCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTGGGCACCGTAACTTAGCTTTCGGTTCA14530.17702651498999125No Hit
CTTTGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGGTT14270.17385880033772713No Hit
GCGCTAAAGCGCGCGACCTATACTTAGCCATTAAGGCAAATGCGAGGCCTTAATGAGTAGGAGGGCGTGGAGGTC14200.17300595408519448No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCCTTTGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG14060.17130026158012915No Hit
TACCAGTTCTGAGTCGGTTGTTCACTGCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTTCAACCCGTCAG13970.17020374496973004No Hit
CCCCTGGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTACCTACA13810.16825438210679827No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCTTTGGGACCATCGCAATGCTTTGTTTTAA13730.1672797006753324No Hit
GGGTAATCCTGTATCAAGGGCGCAATTTGCGCCGCCCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGAC13190.16070060101293768No Hit
TTACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAAGAGCCCAACTTGA13020.15862940297107267No Hit
AAATCACATTGCGTCAACATCCTTTGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC12870.15680187528707415No Hit
ATTTAGGGCTGCATTCCCAAACAACCCGACTCGGCGACAGCGCCTCGTGGAGTAGCAGGGTCCGGGGTCGACGGG12520.1525376440244109No Hit
CCCTGGAATCAGATTAACTGGAGATAGGGTCCAGCGACTGGGAAAGCATCGCACGTCTCGCGGTGTCTGGTGCGC11630.14169431309935293No Hit
GTACATGGGTTGACCTCAGATCAGACAAGATTACCCGCCGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAAC11460.13962311505748792No Hit
GTTGGGCACCGTAACTTAGCTTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAAC11320.13791742255242265No Hit
GAGTAGGAGGGCGTGGAGGTCGTGGCGAAGCCTGGGGCGTGAGCCTGGGTAGAACGGCCTCTAGTGCAGATCTTG11130.13560255415269115No Hit
GTGTCTGGTGCGCCCCTGACGGCCCTTGAAAATTCGGAGGAGCGGATACCGATCGCGCTCGGTCGTACTCATAAC11020.13426236718442555No Hit
CTTTAGCGCCATCCATTTTCGGGGCTAATTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT10880.13255667467936028No Hit
GCCTTAATGAGTAGGAGGGCGTGGAGGTCGTGGCGAAGCCTGGGGCGTGAGCCTGGGTAGAACGGCCTCTAGTGC10860.1323130043214938No Hit
GTATCAAGGGCGCAATTTGCGCCGCCCATCGAAAGGGAATCGGGTTAATATTCCCGAACCGGGACGCGGCGGCTG10800.13158199324789438No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10640.12963263038496262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCAAACAACCCGACTCGGCGACAGCGCCTCGTGGAGTAGCAGGGTCCGGGGTCGACGGGGCTCTCACCCTCT10620.12938896002709616No Hit
GTTGTTCACTGCCCAGGGAAAGCCAGCAAGCCAGCATTCCACGCCCTTCAACCCGTCAGCACGTGGCAGTCCGCC10550.1285361137745635No Hit
AACCTAGATCCCACAAAGGGTGTTGATTGATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG10320.12573390465909906No Hit
CCCCTGACGGCCCTTGAAAATTCGGAGGAGCGGATACCGATCGCGCTCGGTCGTACTCATAACCGCATCAGGTCT10150.12366270661723408No Hit
GTGTAACAACTCACCTGCCGAATCAATTAGCCCCGAAAATGGATGGCGCTAAAGCGCGCGACCTATACTTAGCCA10150.12366270661723408No Hit
TTCCACGCCCTTCAACCCGTCAGCACGTGGCAGTCCGCCCTCGCCGCGTGAGCAGCTCGGGCAGACCACCAACAG10140.12354087143830084No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAGTATTTAGCCTTAGATGGAATTTACCACCCATTTAGGGCTGCATTCC10070.12268802518576818No Hit
ACCCAGGCTCACGCCCCAGGCTTCGCCACGACCTCCACGCCCTCCTACTCATTAAGGCCTCGCATTTGCCTTAAT10030.12220068447003525No Hit
GTTATGAGTACGACCGAGCGCGATCGGTATCCGCTCCTCCGAATTTTCAAGGGCCGTCAGGGGCGCACCAGACAC9970.12146967339643584No Hit
ATACAGGATTACCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTT9760.11891113463883787No Hit
CCTCAGATCAGACAAGATTACCCGCCGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAACTAACAAGGATTCC9630.11732727731270581No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAATTAGCCCCGAAAATGGATGGCGCTAAAGCGCGCGACCTA9110.11099184800817759No Hit
ATACTTAGCCATTAAGGCAAATGCGAGGCCTTAATGAGTAGGAGGGCGTGGAGGTCGTGGCGAAGCCTGGGGCGT8900.10843330925057962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)