FastQCFastQC Report
Wed 8 May 2024
EGAF00002153374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767646
Total Bases57.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92171.200683648452542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48200.6278935863666325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.4737860941110877No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28020.365011997717698Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26170.34091234761856376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.23630683935042976No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12690.16531057284216943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGAGACAGTGCCCAAATCATTACGCCTTTCGTGCGGGTCGGAACTTA12430.1619235949903992No Hit
TTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTTACGATTTAGC11370.14811514682548987No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9610.12518791213658378Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.11554805209693009No Hit
GTTGAGACAGTGCCCAAATCATTACGCCTTTCATGCGGGTCAGAACTTAC8640.1125518793819026No Hit
TTCCAGCACCGGGCAGGCGTCAGCTCGTATACGTCATCTTTCGATTTAGC8630.11242161100298836No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8510.11085839045601747Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA8430.10981624342470356No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8220.10708060746750456Clontech SMART CDS Primer II A (96% over 26bp)
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT7850.10226067744767771No Hit
GGCCTATTCACTGCGGCTGACTTAAAGTCAGCACCCCTTCTCCCGAAGTT7770.10121853041636379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)