FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161886
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36642.2633211025042312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22691.4016035975933683No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17601.087184809063168Illumina PCR Primer Index 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.880249064156258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.6313084516264532No Hit
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4970.3070061648320423Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4620.28538601237908157Illumina PCR Primer Index 2 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.26870760905822616No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.2507937684543444No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.2254673041523047No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.17604981283125162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2250.13898669434046182No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)