FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420328
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116232.765221446108753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71861.7096172512894692No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA57081.3579870957918578Illumina PCR Primer Index 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55391.3177804000685178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.726337526883767No Hit
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA19590.46606459717173254Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA18130.4313298186178413Illumina PCR Primer Index 2 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.24885327648883732No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.17890790049675492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT7510.17866999105460496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6970.1658228811785082No Hit
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA6590.15678232237681047Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA6050.14393521250071373Illumina PCR Primer Index 2 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.14226984640566415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.1372737481205154Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT5410.12870900820311756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT5330.12680573266591805No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.12109590605431947No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.11800308330637026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT4390.10444224510382369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT4210.10015987514512475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)