FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476921
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72031.5103130287825446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.863874729777049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.8166971049712636No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA33570.7038901621023188Illumina PCR Primer Index 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.4038404683375234No Hit
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA9140.191645995877724Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8240.17277494595540982Illumina PCR Primer Index 2 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.1209843978352809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT5000.10483916623507876No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.10190366958049656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)