EGAF00002153416 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00002153416
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104533
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	15951	15.259296107449321	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	5650	5.404991725101165	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	5106	4.884581902365761	Illumina PCR Primer Index 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA	2002	1.915184678522572	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA	1923	1.8396104579415113	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	921	0.8810614829766676	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA	714	0.683037892340218	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC	693	0.6629485425655056	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA	621	0.5940707719093492	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	606	0.5797212363559833	TruSeq Adapter, Index 2 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA	500	0.4783178517788641	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA	496	0.4744913089646332	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG	478	0.4572718663005941	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA	413	0.3950905455693417	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA	380	0.36352156735193675	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	372	0.3558684817234749	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC	333	0.31855968928472345	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	302	0.28890398247443394	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA	296	0.28316416825308754	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG	288	0.2755110826246257	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA	272	0.2602049113677021	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG	271	0.2592482756641443	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA	261	0.24968191862856703	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT	251	0.24011556159298977	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA	249	0.23820229018587433	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	247	0.23628901877875888	TruSeq Adapter, Index 2 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA	232	0.22193948322539295	Illumina PCR Primer Index 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	230	0.2200262118182775	TruSeq Adapter, Index 2 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA	215	0.20567667626491154	Illumina PCR Primer Index 2 (96% over 29bp)
NTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	202	0.19324041211866108	Illumina PCR Primer Index 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	190	0.18176078367596837	TruSeq Adapter, Index 2 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA	187	0.17889087656529518	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA	182	0.17410769804750653	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA	174	0.1664546124190447	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA	170	0.1626280696048138	Illumina PCR Primer Index 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA	169	0.16167143390125607	Illumina PCR Primer Index 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA	165	0.15784489108702515	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA	164	0.15688825538346743	TruSeq Adapter, Index 2 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA	163	0.1559316196799097	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAG	163	0.1559316196799097	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAA	155	0.14827853405144786	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAC	154	0.14732189834789014	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA	153	0.14636526264433242	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAA	149	0.1425387198301015	Illumina PCR Primer Index 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	147	0.14062544842298605	No Hit
TTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAA	145	0.1387121770158706	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA	129	0.12340600575894693	Illumina PCR Primer Index 2 (96% over 29bp)
CATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAA	129	0.12340600575894693	Illumina PCR Primer Index 2 (96% over 29bp)
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTT	123	0.11766619153760058	Illumina PCR Primer Index 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA	121	0.1157529201304851	Illumina PCR Primer Index 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAC	119	0.11383964872336966	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAAAA	118	0.11288301301981192	Illumina PCR Primer Index 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAG	115	0.11001310590913875	Illumina PCR Primer Index 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAA	114	0.10905647020558103	Illumina PCR Primer Index 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAA	110	0.1052299273913501	Illumina PCR Primer Index 2 (96% over 29bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTGCATGTATCTCGTATGCCGTCTTCTGCTTG	105	0.10044674887356148	Illumina PCR Primer Index 2 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content