FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104533
Sequences flagged as poor quality0
Sequence length75
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGTTGCAAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA6430.6151167573876192Illumina Single End PCR Primer 1 (100% over 29bp)
ATACACATCTGACGCTGCCGACGAGTTGCAAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAA2320.22193948322539295Illumina Single End PCR Primer 1 (100% over 29bp)
CATCTGACGCTGCCGACGAGTTGCAAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA1210.1157529201304851Illumina Single End PCR Primer 1 (100% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)