FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209868
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50122.388167800712829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31501.5009434501686774No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA28311.3489431452150875RNA PCR Primer, Index 34 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.9467855985667181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.7056816665713687No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8170.3892923170754951RNA PCR Primer, Index 34 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.3454552385308861No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.27636419082470887No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.2735052509196257No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4990.23776850210608572RNA PCR Primer, Index 41 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.21823241275468389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2900.13818209541235443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2780.13246421560218802No Hit
ACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2660.12674633579202166RNA PCR Primer, Index 34 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT2240.10673375645643929No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2170.10339832656717557RNA PCR Primer, Index 34 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)