FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242505
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43651.7999628873631472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27661.1405950392775408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.7735922970660398No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18330.7558607039030123RNA PCR Primer, Index 34 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.4911238943526937No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.2498917548091792No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5010.20659367848085605RNA PCR Primer, Index 34 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.20082060163707965No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1835013711057504No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3810.1571101626770582RNA PCR Primer, Index 41 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.14350219583101378No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2830.11669862477062327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2570.10597719634646709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)