FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205153
Sequences flagged as poor quality0
Sequence length75
%GC24

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49762.425506816863512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31471.5339770805203923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21121.0294755621414262No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18860.9193138779350046RNA PCR Primer, Index 34 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.7296992975974029No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.3602189585333873No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.29441441265787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.279303739160529No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5260.25639400837423776RNA PCR Primer, Index 34 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.24128333487689674No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3050.14866952957061316RNA PCR Primer, Index 41 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2370.11552353609257482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2370.11552353609257482No Hit
ACACATCTCCGAGCCCACGAGACCTCATGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2110.10285006799803074RNA PCR Primer, Index 34 (96% over 30bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10090030367579318No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)